KCC2/SLC12A5 Polyklonaler Antikörper

KCC2/SLC12A5 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 28724-1-AP

Synonyme

hKCC2, K Cl cotransporter 2, KCC2, KCC2/SLC12A5, KIAA1176, Neuronal K Cl cotransporter, SLC12A5

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  WB analysis using 28724-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 28724-1-AP (KCC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 28724-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 28724-1-AP (KCC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 28724-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 28724-1-AP (KCC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 28724-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28724-1-AP (KCC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 28724-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28724-1-AP (KCC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse cerebellum using 28724-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using KCC2/SLC12A5 antibody (28724-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse cerebellum using 28724-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using KCC2/SLC12A5 antibody (28724-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche Detektion in IHC: Rattenhirngewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Cerebellum-Gewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

28724-1-AP bindet in WB, IHC, IF-P, ELISA KCC2/SLC12A5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KCC2/SLC12A5 fusion protein Ag30203
• Vollständiger Name: solute carrier family 12 (potassium-chloride transporter), member 5
• Beobachtetes Molekulargewicht: 130-150 kDa
• GenBank-Zugangsnummer: BC132670
• Gene symbol: KCC2
• Gene ID (NCBI): 57468
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KCC2, encoded by SLC12A5, is a neuron-specific K+-Cl- cotransporter that is essential for Cl- homeostasis and inhibitory synaptic transmission in brain. KCC2 is expressed at high levels in neurons throughout the nervous system. KCC2 deletion caused animal death. Alterations of KCC2 have been linked to neuropathic pain, temporal lobe epilepsy, and other diseases.

Publikationen

Species	Application	Title
human	WB	Cell Death Dis; The NTE domain of PTENα/β promotes cancer progression by interacting with WDR5 via its SSSRRSS motif; Authors - Xiaolei Huang