Kir6.2 Polyklonaler Antikörper

Kir6.2 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 16920-1-AP

Synonyme

BIR, HHF2, IKATP, KCNJ11, KIR6.2, PHHI, TNDM3

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  WB analysis of rat heart using 16920-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 16920-1-AP (Kir6.2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 16920-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16920-1-AP (Kir6.2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16920-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16920-1-AP (KCNJ11 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 16920-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16920-1-AP (KCNJ11 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenherzgewebe, HepG2-Zellen, humanes Herzgewebe, Ratten-Skelettmuskelgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

16920-1-AP bindet in WB, ELISA Kir6.2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Kir6.2 fusion protein Ag10262
• Vollständiger Name: potassium inwardly-rectifying channel, subfamily J, member 11
• Berechnetes Molekulargewicht: 390 aa, 44 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC064497
• Gene symbol: Kir6.2
• Gene ID (NCBI): 3767
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Kir6.2 (also known as BIR or IKATP), encoded by the KCNJ11 gene, is the pore-forming unit of the ATP-sensitive K+ channel, an inward-rectifier potassium ion channel. Kir6.2 is controlled by G-proteins and is found associated with the sulfonylurea receptor (SUR) to constitute the ATP-sensitive K+ channel. The KCNJ11 gene is located at 11p15.1 and has no intron. Mutations in KCNJ11 are a cause of familial PHHI, an autosomal recessive disorder characterized by unregulated ins secretion. Defects in KCNJ11 may also contribute to autosomal dominant non-ins-dependent diabetes mellitus type II (NIDDM), transient neonatal diabetes mellitus type 3 (TNDM3), and permanent neonatal diabetes mellitus (PNDM).

Publikationen

Species	Application	Title
		Life Sci; KATP channels as ROS-dependent modulator of neurotransmitter release at the neuromuscular junctions; Authors - Andrei N Tsentsevitsky
rat	WB	Sci Rep; Glucocorticoid receptor-NECAB1 axis can negatively regulate insulin secretion in pancreatic β-cells; Authors - Haruhide Udagawa
mouse	WB	Int Immunopharmacol; Nicorandil restores endothelial cell Kir6.2 expression to alleviate neuropathic pain in mice after chronic constriction injury; Authors - Minghao Du