KDM1 Polyklonaler Antikörper

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• KD/KO Validated

KDM1 Polyklonal Antikörper für FC, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 20813-1-AP

Synonyme

AOF2, BHC110, KDM1, KDM1A, KIAA0601, LSD1, lysine (K) demethylase 1

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 20813-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 20813-1-AP (KDM1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 20813-1-AP

  IP Result of anti-KDM1 (IP:20813-1-AP, 4ug; Detection:20813-1-AP 1:600) with HeLa cells lysate 2000ug.

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  IHC staining of human lung cancer using 20813-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20813-1-AP (KDM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 20813-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20813-1-AP (KDM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HeLa using 20813-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human KDM1 (20813-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below

Produktinformation

20813-1-AP bindet in WB, IP, IHC, FC, ELISA KDM1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KDM1 fusion protein Ag14778
• Vollständiger Name: lysine (K)-specific demethylase 1
• Berechnetes Molekulargewicht: 876 aa, 95 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC040194
• Gene symbol: KDM1
• Gene ID (NCBI): 23028
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lysine specific demethylase 1 (LSD1/BHC110/KIAA0601/p110b/AOF2/KDM1) is an amine oxidase that catalyzes histone demethylation via a flavin adenine dinucleotide (FAD)-dependent oxidative reaction(PMID:19703393). This protein belongs to the flavin monoamine oxidase family. Its structure and function is conserved from yeast to human and it is typically associated to CoREST, a corepressor protein, and histone deacetylases HDAC1 and HDAC2. Human LSD1 consists of 852 amino acids and comprises an N-terminal SWIRM domain, involved in protein interactions, and a C-terminal amine oxidase domain, which contains an insertion that forms the CoREST interacting site (the so-called tower domain )(PMID:20164337). It has 2 isoforms produced by alternative splicing with the calculated molecular mass of 93-95 kDa and apparent molecular mass of 110 kDa.

Publikationen

Species	Application	Title
		Cancer Res; HBXIP and LSD1 Scaffolded by lncRNA Hotair Mediate Transcriptional Activation by c-Myc.; Authors - Yinghui Li
human	WB	EMBO J; The chromatin remodeler SRCAP promotes self-renewal of intestinal stem cells.; Authors - Buqing Ye
human	WB	Int J Mol Sci; The Lysine Specific Demethylase-1 Negatively Regulates the COL9A1 Gene in Human Articular Chondrocytes.; Authors - Anne-Laure Durand ; KD Validated
human,mouse	WB	J Biol Chem; The Deubiquitinase USP28 Maintains the Expression of the Transcription Factor MYCN and Is Essential in Neuroblastoma Cells; Authors - Junjun Li
human	WB	Cancer Cell Int; 4,5-Dimethoxycanthin-6-one is a novel LSD1 inhibitor that inhibits proliferation of glioblastoma cells and induces apoptosis and pyroptosis.; Authors - Wei Li
mouse	WB	Adv Sci (Weinh); PWWP2B Fine-Tunes Adipose Thermogenesis by Stabilizing HDACs in a NuRD Subcomplex.; Authors - Linyu Yan