KEAP1 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

KEAP1 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(16)

CloneNo.

5O18

Kat-Nr. : 80744-1-RR

Synonyme

KLHL19, KIAA0132, Kelch-like ECH-associated protein 1, KEAP 1, 5O18

---

• 

  WB analysis of HEK-293 using 80744-1-RR

  WB result of KEAP1 antibody (80744-1-RR; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KEAP1 transfected HEK-293 cells.

• 

  WB analysis using 80744-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80744-1-RR (KEAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 80744-1-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 80744-1-RR (KEAP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 80744-1-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 80744-1-RR (KEAP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 80744-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using KEAP1 antibody (80744-1-RR, Clone: 5O18 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 80744-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using KEAP1 antibody (80744-1-RR, Clone: 5O18 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  Affinity and Kinetic Characterization of 80744-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80744-1-RR against Human KEAP1 were performed. The affinity constant is 0.224 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, 4T1-Zellen, HEK-293-Zellen, HeLa-Zellen, K-562-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 14 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

80744-1-RR bindet in WB, IHC, IF/ICC, IP, ELISA KEAP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: KEAP1 fusion protein Ag0779
• Vollständiger Name: kelch-like ECH-associated protein 1
• Berechnetes Molekulargewicht: 624 aa, 70 kDa
• Beobachtetes Molekulargewicht: 55~70 kDa
• GenBank-Zugangsnummer: BC002930
• Gene symbol: KEAP1
• Gene ID (NCBI): 9817
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Kelch-like ECH-associated protein 1 (KEAP1) is a negative regulator of nuclear factor erythroid 2-related factor 2 (Nrf2), a transcription factor governing the antioxidant response.

What is the molecular weight of KEAP1 protein? Are there any isoforms of KEAP1?

The molecular weight of KEAP1 protein is 70 kDa. The KEAP1 gene gives rise only to protein isoforms, but mutations of KEAP1 protein have been found in various cancer types.

What is the subcellular localization of KEAP1?

KEAP1 resides in the cytoplasm, where it binds to Nrf2, targeting it for degradation and preventing translocation of Nrf2 to the nucleus.

How does KEAP1 control Nrf2 levels? Is KEAP1 post-translationally modified?

KEAP1 is rich in reactive cysteine residues, whose thiol groups play a role in binding to CUL3 and the polyubiquitination of Nrf2, which leads to degradation of Nrf2 via the proteasome system. During oxidative stress, electrophiles and reactive oxygen species (ROS) modify the KEAP1 thiol groups, reducing the affinity of KEAP1 to CUL3 and the stabilization of Nrf2. Nrf2 then translocates to the nucleus, where it binds to the antioxidant responsive elements (AREs) and induces the expression of antioxidant proteins (PMID: 16354693).

How to measure oxidative stress using KEAP1 and Nrf2 proteins as a readout

Under basal conditions (unstressed cells), a detectable KEAP1 protein level is observed. Oxidative stress modifies KEAP1 protein activity by increasing the Nrf2 protein levels. This can be measured, for example, using western blotting (PMID: 27697860). KEAP1 protein levels are not altered by oxidative stress.

What is the role of the KEAP1-Nrf2 pathway in health and disease?

The KEAP-Nrf2 pathway plays a vital role in redox homeostasis and cryoprotection. Inhibition of KEAP1 activity leads to the activation of Nrf2 and increase the response to oxidative stress and anti-inflammatory effects (PMID: 29717933). The activation of Nrf2 can be beneficial in the case of metabolic diseases, such as diabetes, as well as neurodegenerative diseases such as Parkinson's and Alzheimer's diseases. However, the increased activation of Nrf2 is also known to promote tumor growth and metastasis. Mutations in both KEAP1 and Nrf2 were found in various solid tumor types.

Publikationen

Species	Application	Title
mouse	WB	Sci Total Environ; Effects of chronic low-level lead (Pb) exposure on cognitive function and hippocampal neuronal ferroptosis: An integrative approach using bioinformatics analysis, machine learning, and experimental validation; Authors - Yingsi Cao
mouse	WB	Clin Immunol; Potential therapeutic effect of dimethyl fumarate on Treg/Th17 cell imbalance in biliary atresia; Authors - Mengting Liu
mouse	WB	Phytomedicine; Xanthohumol ameliorates drug-induced hepatic ferroptosis via activating Nrf2/xCT/GPX4 signaling pathway; Authors - Yanyan Deng ; KD Validated
mouse	WB,IP,IF	Metab Brain Dis; Inhibitor of apoptosis stimulating protein of p53 protects against MPP+-induced neurotoxicity of dopaminergic neurons; Authors - Lei Chen
mouse	WB	Acta Pharmacol Sin; Artemisinin inhibits neuronal ferroptosis in Alzheimer's disease models by targeting KEAP1; Authors - Peng-Xi Deng
rat	WB	Biomed Pharmacother; miR-200a-3p-enriched MSC-derived extracellular vesicles reverse erectile function in diabetic rats by targeting Keap1; Authors - Jing Zhang