Ki-67 Polyklonaler Antikörper

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• KD/KO Validated

Ki-67 Polyklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

Publikationen(1)

Kat-Nr. : CL594-27309

Synonyme

KI67, MKI67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Ki 67

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  IF Staining of HeLa using CL594-27309

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 Ki-67 antibody (CL594-27309) at dilution of 1:200, CL488-Phalloidin (green).

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  IF Staining of HeLa using CL594-27309

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 KI67 antibody (CL594-27309) at dilution of 1:200.

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  IF Staining of HeLa using CL594-27309

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 KI67 antibody (CL594-27309) at dilution of 1:200.

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  FC experiment of HeLa using CL594-27309

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human KI67 (CL594-27309) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of Ramos using CL594-27309

  1x10^6 Ramos cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human KI67 (CL594-27309)(red), or 0.8 ug CoraLite®594-conjugated Rabbit IgG control Rabbit PolyAb (CL594-30000) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): Ramos-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

CL594-27309 bindet in IF/ICC, FC (Intra) Ki-67 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Ki-67 fusion protein Ag26266
• Vollständiger Name: antigen identified by monoclonal antibody Ki-67
• Berechnetes Molekulargewicht: 359 kDa
• GenBank-Zugangsnummer: NM_002417
• Gene symbol: KI67
• Gene ID (NCBI): 4288
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.

Publikationen

Species	Application	Title
human	IF	Oncol Rep; Zinc finger protein 180 induces an apoptotic phenotype by activating METTL14 transcriptional activity in colorectal cancer; Authors - Liang Xu