CEP44 Polyklonaler Antikörper

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• KD/KO Validated

CEP44 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 24457-1-AP

Synonyme

Centrosomal protein of 44 kDa, HBV PreS1-transactivated protein 3, PS1TP3

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  WB analysis using 24457-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24457-1-AP (CEP44 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 24457-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24457-1-AP (CEP44 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 24457-1-AP

  IP result of anti-CEP44 (IP:24457-1-AP, 4ug; Detection:24457-1-AP 1:3000) with HeLa cells lysate 1640 ug.

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  IHC staining of mouse brain using 24457-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24457-1-AP (CEP44 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 24457-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24457-1-AP (CEP44 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 24457-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 24457-1-AP (CEP44 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 24457-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 24457-1-AP (CEP44 antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of MDCK using 24457-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CEP44 antibody (24457-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, MCF-7-Zellen, Maushodengewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MDCK-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Produktinformation

24457-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA CEP44 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CEP44 fusion protein Ag19961
• Vollständiger Name: KIAA1712
• Berechnetes Molekulargewicht: 390 aa, 44 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC038667
• Gene symbol: CEP44
• Gene ID (NCBI): 80817
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CEP44, also named as KIAA1712 and PS1TP3, localizes to centrosomes, spindle poles, and weakly in midbody. It is a 44 kDa centrosome protein.

Publikationen

Species	Application	Title
human	IF	J Cell Biol; Centrosomal organization of Cep152 provides flexibility in Plk4 and procentriole positioning; Authors - Catherine Sullenberger
human	WB,IF,IP	J Cell Sci; Cep44 functions in centrosome cohesion by stabilizing rootletin; Authors - Delowar Hossain ; KD Validated
human	WB,IF	J Cell Sci; Cep44 functions in centrosome cohesion by stabilizing rootletin.; Authors - Delowar Hossain ; KD Validated
		Mol Biol Cell; "Roles for ELMOD2 and Rootletin in Ciliogenesis".; Authors - Rachel E Turn
mouse	WB	Cell Rep; A program of successive gene expression in mouse one-cell embryos; Authors - Maki Asami
human	IF	Nat Commun; Molecular basis promoting centriole triplet microtubule assembly; Authors - Yutaka Takeda