Eg5 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Eg5 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(23)

Rezensionen (1)

Kat-Nr. : 23333-1-AP

Synonyme

KIF11, KNSL1, Kinesin-related motor protein Eg5, Kinesin-like spindle protein HKSP, Kinesin-like protein KIF11

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  WB analysis using 23333-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23333-1-AP (Eg5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 23333-1-AP

  IP result of anti-Eg5 (IP:23333-1-AP, 4ug; Detection:23333-1-AP 1:1000) with Raji cells lysate 2000ug.

• 

  IHC staining of human lung cancer using 23333-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 23333-1-AP (Eg5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 23333-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 23333-1-AP (Eg5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 23333-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 23333-1-AP (Eg5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate hyperplasia using 23333-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 23333-1-AP (Eg5 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 23333-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 23333-1-AP (Eg5 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human endometrial cancer using 23333-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 23333-1-AP (Eg5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 23333-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Eg5 antibody (23333-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 23333-1-AP

  Immunofluorescent analysis of HepG2 cells using 23333-1-AP (Eg5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, Raji-Zellen
• Erfolgreiche IP: Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Endometriumkarzinomgewebe, humanes Prostatahyperplasie-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 17 publications below
• IHC: See 9 publications below
• IF: See 7 publications below

Produktinformation

23333-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA Eg5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Eg5 fusion protein Ag18695
• Vollständiger Name: kinesin family member 11
• Berechnetes Molekulargewicht: 1056 aa, 119 kDa
• Beobachtetes Molekulargewicht: 120-130 kDa
• GenBank-Zugangsnummer: BC136474
• Gene symbol: Eg5
• Gene ID (NCBI): 3832
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Eg5 is a plus-end-directed microtubule motor encoded by KIF11 gene. It is an essential mitotic kinesin that plays a pivotal role in the formation and maintenance of bipolar spindle during early prometaphase. Eg5 has been implicated in tumourigenesis, and its overexpression has been found in various cancer tissues.

Publikationen

Species	Application	Title
mouse	IF	Br J Cancer; Albendazole inhibits colon cancer progression and therapy resistance by targeting ubiquitin ligase RNF20; Authors - Iram Fatima
human	IF	Cells; Microtubule Association of EML4-ALK V3 Is Key for the Elongated Cell Morphology and Enhanced Migration Observed in V3 Cells; Authors - Savvas Papageorgiou
mouse	IHC	Int J Oncol; Cyclovirobuxine D inhibits growth and progression of non‑small cell lung cancer cells by suppressing the KIF11‑CDC25C‑CDK1‑CyclinB1 G2/M phase transition regulatory network and the NFκB/JNK signaling pathway; Authors - Ting Xue
human	IHC	Int J Cancer; Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma.; Authors - Fangqiu Fu
human	WB	Int J Biol Sci; KIF11 promotes cell proliferation via ERBB2/PI3K/AKT signaling pathway in gallbladder cancer.; Authors - Dang Wei
human	WB	Mol Carcinog; Kinesin family member 11 is a potential therapeutic target and is suppressed by microRNA-30a in breast cancer.; Authors - Benfang Wang ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Iram (Verified Customer) (01-15-2021)

Eg5 antibody giving a very clean bands and good staining

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:1000 for western and 1:100 for IHC and IF
• Cell Tissue Type: Human colon cancer cells and tissue