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Eg5 Polyklonaler Antikörper
Eg5 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human und mehr (1)
Anwendung
WB, IHC, IF/ICC, IP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 23333-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, Raji-Zellen |
| Erfolgreiche IP | Raji-Zellen |
| Erfolgreiche Detektion in IHC | humanes Lungenkarzinomgewebe, humanes Endometriumkarzinomgewebe, humanes Prostatahyperplasie-Gewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF/ICC | HepG2-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunhistochemie (IHC) | IHC : 1:200-1:800 |
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 6 publications below |
| WB | See 17 publications below |
| IHC | See 9 publications below |
| IF | See 7 publications below |
Produktinformation
23333-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA Eg5 und zeigt Reaktivität mit human
| Getestete Reaktivität | human |
| In Publikationen genannte Reaktivität | human, Maus |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | Eg5 fusion protein Ag18695 |
| Vollständiger Name | kinesin family member 11 |
| Berechnetes Molekulargewicht | 1056 aa, 119 kDa |
| Beobachtetes Molekulargewicht | 120-130 kDa |
| GenBank-Zugangsnummer | BC136474 |
| Gene symbol | Eg5 |
| Gene ID (NCBI) | 3832 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-affinitätsgereinigt |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Eg5 is a plus-end-directed microtubule motor encoded by KIF11 gene. It is an essential mitotic kinesin that plays a pivotal role in the formation and maintenance of bipolar spindle during early prometaphase. Eg5 has been implicated in tumourigenesis, and its overexpression has been found in various cancer tissues.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for Eg5 antibody 23333-1-AP | Protokoll herunterladen |
| IHC protocol for Eg5 antibody 23333-1-AP | Protokoll herunterladenl |
| IF protocol for Eg5 antibody 23333-1-AP | Protokoll herunterladen |
| IP protocol for Eg5 antibody 23333-1-AP | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Br J Cancer Albendazole inhibits colon cancer progression and therapy resistance by targeting ubiquitin ligase RNF20 | ||
Cells Microtubule Association of EML4-ALK V3 Is Key for the Elongated Cell Morphology and Enhanced Migration Observed in V3 Cells | ||
Int J Oncol Cyclovirobuxine D inhibits growth and progression of non‑small cell lung cancer cells by suppressing the KIF11‑CDC25C‑CDK1‑CyclinB1 G2/M phase transition regulatory network and the NFκB/JNK signaling pathway | ||
Int J Cancer Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma. | ||
Int J Biol Sci KIF11 promotes cell proliferation via ERBB2/PI3K/AKT signaling pathway in gallbladder cancer. | ||
Mol Carcinog Kinesin family member 11 is a potential therapeutic target and is suppressed by microRNA-30a in breast cancer.
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Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Iram (Verified Customer) (01-15-2021) | Eg5 antibody giving a very clean bands and good staining
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