KIF17 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

KIF17 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 14615-1-AP

Synonyme

Kinesin-like protein KIF17, KIF3X, KIF3-related motor protein, KIAA1405

---

• 

  WB analysis using 14615-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14615-1-AP (KIF17 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14615-1-AP

  IP result of anti-KIF17 (IP:14615-1-AP, 4ug; Detection:14615-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human gliomas using 14615-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14615-1-AP (KIF17 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 14615-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14615-1-AP (KIF17 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14615-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14615-1-AP (KIF17 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14615-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14615-1-AP (KIF17 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 14615-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14615-1-AP (KIF17 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 14615-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14615-1-AP (KIF17 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of COS-7 using 14615-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed COS-7 cells using 14615-1-AP (KIF17 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of COS-7 using 14615-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed COS-7 cells using KIF17 antibody (14615-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Hirngewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: COS-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

14615-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA KIF17 und zeigt Reaktivität mit Affe, human, Maus, Ratten

• Getestete Reaktivität: Affe, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KIF17 fusion protein Ag6217
• Vollständiger Name: kinesin family member 17
• Berechnetes Molekulargewicht: 115 kDa
• Beobachtetes Molekulargewicht: 170 kDa
• GenBank-Zugangsnummer: BC065927
• Gene symbol: KIF17
• Gene ID (NCBI): 57576
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Dev Cell; ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.; Authors - Jie Ran
human	WB,IF,IP	Neurosci Bull; KIF17 Modulates Epileptic Seizures and Membrane Expression of the NMDA Receptor Subunit NR2B.; Authors - Yan Liu
human	WB,IHC,IF,CoIP	Cancer Lett; KIF17 maintains the epithelial phenotype of breast cancer cells and curbs tumour metastasis; Authors - Tianfu Li ; KD Validated
human	WB	J Biol Chem; Structural basis for Rab23 activation and a loss-of-function mutation in Carpenter Syndrome; Authors - Yat Yin Chau