KIF18A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

KIF18A Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(16)

Kat-Nr. : 19245-1-AP

Synonyme

PPP1R99, OK/SW-cl.108, MS-KIF18A, Kinesin-like protein KIF18A

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  WB analysis of Jurkat using 19245-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19245-1-AP (KIF18A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19245-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19245-1-AP (KIF18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 19245-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 19245-1-AP (KIF18A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 19245-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 19245-1-AP (KIF18A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 19245-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19245-1-AP (KIF18A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 19245-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19245-1-AP (KIF18A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 19245-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19245-1-AP (KIF18A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 19245-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19245-1-AP (KIF18A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: human colon cancer, humanes Mammakarzinomgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 8 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Produktinformation

19245-1-AP bindet in WB, IHC, IF, IP, ELISA KIF18A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KIF18A fusion protein Ag6166
• Vollständiger Name: kinesin family member 18A
• Berechnetes Molekulargewicht: 102 kDa
• Beobachtetes Molekulargewicht: 102 kDa
• GenBank-Zugangsnummer: BC048347
• Gene symbol: KIF18A
• Gene ID (NCBI): 81930
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KIF18A belongs to the Kinesin family. KIF18A binds to microtubules and moves along them using energy from ATP hydrolysis. KIF18A is related to the localization of membrane organelles and protein complexes, as well as the maintenance of microtubule dynamics and mitosis. KIF18A expression is low in normal tissues and high in solid tumors, including breast cancer, hepatocellular carcinoma and lung adenocarcinoma, colorectal cancer, renal cell carcinoma, and head and neck squamous cell carcinoma. The molecular weight of KIF18A is 102 kDa (PMID: 36830695).

Publikationen

Species	Application	Title
human	IF	Proc Natl Acad Sci U S A; Maternal genetic variants in kinesin motor domains prematurely increase egg aneuploidy; Authors - Leelabati Biswas
human	IHC	Oncotarget; High KIF18A expression correlates with unfavorable prognosis in primary hepatocellular carcinoma.; Authors - Weijia Liao
human	WB	Oncotarget; Exclusive destruction of mitotic spindles in human cancer cells.; Authors - Leonid Visochek
mouse	IF	Sci Rep; PMID: 29229923; Authors - Sara Bizzotto
human	WB,IHC	Front Genet; Prognostic Biomarker KIF18A and Its Correlations With Immune Infiltrates and Mitosis in Glioma.; Authors - Bing-Yan Tao
human	WB	Dis Markers; Kinesin Family Member 18A (KIF18A) Contributes to the Proliferation, Migration, and Invasion of Lung Adenocarcinoma Cells In Vitro and In Vivo.; Authors - Fu-Tao Chen ; KD Validated