KIF18A Polyklonaler Antikörper

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• KD/KO Validated

KIF18A Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 19245-1-AP

Synonyme

KIF18A, kinesin family member 18A, Kinesin like protein KIF18A, Marrow stromal KIF18A, MS KIF18A

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 19245-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19245-1-AP (KIF18A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19245-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19245-1-AP (KIF18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 19245-1-AP

  IP Result of anti-KIF18A (IP:19245-1-AP, 4ug; Detection:19245-1-AP 1:500) with Jurkat cells lysate 2400ug.

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  IHC staining of human colon cancer using 19245-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19245-1-AP (KIF18A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 19245-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19245-1-AP (KIF18A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 19245-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19245-1-AP (KIF18A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 19245-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19245-1-AP (KIF18A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 19245-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19245-1-AP (KIF18A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 19245-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19245-1-AP (KIF18A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Lebergewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 7 publications below
• IF: See 2 publications below

Produktinformation

19245-1-AP bindet in WB, IP, IHC, IF, ELISA KIF18A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KIF18A fusion protein Ag6166
• Vollständiger Name: kinesin family member 18A
• Berechnetes Molekulargewicht: 102 kDa
• Beobachtetes Molekulargewicht: 102 kDa
• GenBank-Zugangsnummer: BC048347
• Gene symbol: KIF18A
• Gene ID (NCBI): 81930
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KIF18A belongs to the Kinesin family. KIF18A binds to microtubules and moves along them using energy from ATP hydrolysis. KIF18A is related to the localization of membrane organelles and protein complexes, as well as the maintenance of microtubule dynamics and mitosis. KIF18A expression is low in normal tissues and high in solid tumors, including breast cancer, hepatocellular carcinoma and lung adenocarcinoma, colorectal cancer, renal cell carcinoma, and head and neck squamous cell carcinoma. The molecular weight of KIF18A is 102 kDa (PMID: 36830695).

Publikationen

Species	Application	Title
human	IHC	Oncotarget; High KIF18A expression correlates with unfavorable prognosis in primary hepatocellular carcinoma.; Authors - Weijia Liao
human	WB	Oncotarget; Exclusive destruction of mitotic spindles in human cancer cells.; Authors - Leonid Visochek
mouse	IF	Sci Rep; PMID: 29229923; Authors - Sara Bizzotto
human	WB,IHC	Front Genet; Prognostic Biomarker KIF18A and Its Correlations With Immune Infiltrates and Mitosis in Glioma.; Authors - Bing-Yan Tao
human	WB	Dis Markers; Kinesin Family Member 18A (KIF18A) Contributes to the Proliferation, Migration, and Invasion of Lung Adenocarcinoma Cells In Vitro and In Vivo.; Authors - Fu-Tao Chen ; KD Validated
mouse	WB,IF	Cell Div; Kif18a regulates Sirt2-mediated tubulin acetylation for spindle organization during mouse oocyte meiosis.; Authors - Feng Tang ; KD Validated