KIF20A Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

KIF20A Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

CloneNo.

2A8E3

Kat-Nr. : 67190-1-Ig

Synonyme

FLJ21151, GG10_2, KIF20A, kinesin family member 20A, Kinesin like protein KIF20A, MKLP2, RAB6KIFL, Rabkinesin 6

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Galerie der Validierungsdaten

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  WB analysis using 67190-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67190-1-Ig (KIF20A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis of HeLa using 67190-1-Ig

  WB result of KIF20A antibody (67190-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF20A transfected HeLa cells.

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  IHC staining of human breast cancer using 67190-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67190-1-Ig (KIF20A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67190-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67190-1-Ig (KIF20A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 67190-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67190-1-Ig (KIF20A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 67190-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67190-1-Ig (KIF20A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67190-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 67190-1-Ig (KIF20A antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HEK-293-Zellen, HeLa-Zellen, HL-60-Zellen, Jurkat-Zellen, NIH/3T3-Zellen, PC-12-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Produktinformation

67190-1-Ig bindet in WB, IHC, IF, ELISA KIF20A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: KIF20A fusion protein Ag8916
• Vollständiger Name: kinesin family member 20A
• Berechnetes Molekulargewicht: 890 aa, 100 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC012999
• Gene symbol: KIF20A
• Gene ID (NCBI): 10112
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KIF20A, also known as RAB6KIFL or MKlp2, is a kinesin originally identified as a binding partner for the rab6 GTPase involved in protein transport at the Golgi apparatus. KIF20A belongs to a large family of motor proteins that accumulate in mitotic cells and is required for normal cell division. KIF20A is thought to be involved in carcinogenesis. In normal tissues KIF20A is almost undetectable while it is overexpressed in various cancers. KIF20A has been identified as a novel promising candidate for anticancer immunotherapy.

Publikationen

Species	Application	Title
human	IF	Cancer Res; Proteomic mapping and targeting of mitotic pericentriolar material in tumors bearing centrosome amplification.; Authors - Bingteng Xie
mouse,human	WB,IF	Genomics; Upregulation of the key biomarker kinesin family member 20A (KIF20A) is associated with the pulmonary artery hypertension; Authors - Linqing Li
mouse	IF	Res Sq; An oocyte meiotic midbody cap is required for developmental competence in mice; Authors - Karen Schindler
mouse	IF	Nat Commun; An oocyte meiotic midbody cap is required for developmental competence in mice; Authors - Gyu Ik Jung
human	WB	Mol Cancer Res; Overlaid transcriptome and proteome analyses identify mitotic kinesins as important targets of arylsulfonamide-mediated RBM39 degradation; Authors - Seemon Coomar
human	WB	Mol Cancer Res; Overlaid Transcriptional and Proteome Analyses Identify Mitotic Kinesins as Important Targets of Arylsulfonamide-Mediated RBM39 Degradation; Authors - Seemon Coomar