KIF22 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

KIF22 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 13403-1-AP

Synonyme

KID, KIF22, kinesin family member 22, Kinesin like protein 4, Kinesin like protein KIF22, KNSL4, OBP, OBP 1, OBP 2

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Galerie der Validierungsdaten

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  WB analysis using 13403-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13403-1-AP (KIF22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 13403-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13403-1-AP (KIF22 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 13403-1-AP

  IP Result of anti-KIF22 (IP:13403-1-AP, 4ug; Detection:13403-1-AP 1:300) with HeLa cells lysate 4000ug.

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  IHC staining of human cervical cancer using 13403-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13403-1-AP (KIF22 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human cervical cancer using 13403-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13403-1-AP (KIF22 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human breast cancer using 13403-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13403-1-AP (KIF22 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13403-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13403-1-AP (KIF22 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, humanes Herzgewebe, MKN-45-Zellen, T-47D-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

13403-1-AP bindet in WB, IP, IHC, IF, ELISA KIF22 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KIF22 fusion protein Ag4254
• Vollständiger Name: kinesin family member 22
• Berechnetes Molekulargewicht: 665 aa, 73 kDa
• Beobachtetes Molekulargewicht: 68-73 kDa
• GenBank-Zugangsnummer: BC028155
• Gene symbol: KIF22
• Gene ID (NCBI): 3835
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KIF22, also known as KID (kinesin-like DNA-binding protein), is a kinesin-like DNA-binding protein known to be involved in chromosome movement during mitosis. KIF22 is a nuclear protein regulated by SIAH-1, whose level fluctuate in a cell cycle-dependent manner, increasing during pre-mitotic phases and greatly decreasing during mitosis. KIF22 localizes to the nucleus of the interphase cells upon entry into mitosis and displays some punctate cytoplasmic staining. Defects in KIF22 are the cause of spondyloepimetaphyseal dysplasia with joint laxity, type 2 (SEMDJL2).

Publikationen

Species	Application	Title
mouse	IF	Sci Rep; PMID: 29229923; Authors - Sara Bizzotto
human	WB	Proteomics; Genistein-induced mitotic arrest of gastric cancer cells by downregulating KIF20A, a proteomics study.; Authors - Yan Guang-Rong GR
human	WB,IHC	Chin Med J (Engl); Effect of KIF22 on promoting proliferation and migration of gastric cancer cells via MAPK-ERK pathways.; Authors - Ze-Yuan Yu ; KD Validated