KIF23 Polyklonaler Antikörper

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• KD/KO Validated

KIF23 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Rezensionen (1)

Kat-Nr. : 28587-1-AP

Synonyme

MKLP1, KNSL5

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  WB analysis using 28587-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28587-1-AP (KIF23 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 28587-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28587-1-AP (KIF23 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 28587-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28587-1-AP (KIF23 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IF Staining of HepG2 using 28587-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KIF23 antibody (28587-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, U-87 MG-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

28587-1-AP bindet in WB, IHC, IF/ICC, ELISA KIF23 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KIF23 fusion protein Ag29346
• Vollständiger Name: kinesin family member 23
• Berechnetes Molekulargewicht: 110 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: NM_138555
• Gene symbol: KIF23
• Gene ID (NCBI): 9493
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KIF23, also known as KNSL5 and MKLP1, is a member of the kinesin-like protein family. It plays a crucial role in cytokinesis, the final stage of cell division. It is involved in the formation of the central spindle, which is essential for the proper separation of the cytoplasm and the formation of the cleavage furrow. KIF23 is also involved in the organization and transport of intracellular vesicles and organelles. Mutations in KIF23 have been associated with congenital dyserythropoietic anemia type III(PMID: 23570799).

Publikationen

Species	Application	Title
		Cell Biosci; TPM2 attenuates progression of prostate cancer by blocking PDLIM7-mediated nuclear translocation of YAP1; Authors - Zonglong Wu
human	IF	PLoS Biol; The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization.; Authors - Umut Batman
human	WB,IHC	Front Cell Dev Biol; Identification of KIF23 as a Prognostic Biomarker Associated With Progression of Clear Cell Renal Cell Carcinoma.; Authors - Zonglong Wu ; KD Validated
human	WB	Front Immunol; HBx Mediated Increase of DDX17 Contributes to HBV-Related Hepatocellular Carcinoma Tumorigenesis.; Authors - Mei-Ling Dong
human	WB	Toxicol Appl Pharmacol; Kinesin family member 23 exerts a protumor function in breast cancer via stimulation of the Wnt/β-catenin pathway.; Authors - Xin He ; KD Validated
	WB	Cell Rep; Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals.; Authors - Ana C Almeida ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wissem (Verified Customer) (07-30-2024)

antibody works well to detect KIF23 in U2OS cell extracts.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10,000