KIF26B Polyklonaler Antikörper

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• KD/KO Validated

KIF26B Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(14)

Rezensionen (1)

Kat-Nr. : 17422-1-AP

Synonyme

Kinesin like protein KIF26B, Kinesin-like protein KIF26B

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  WB analysis of MDCK using 17422-1-AP

  MDCK cells were subjected to SDS PAGE followed by western blot with 17422-1-AP (KIF26B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17422-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17422-1-AP (KIF26B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 17422-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17422-1-AP (KIF26B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 17422-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17422-1-AP (KIF26B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 17422-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17422-1-AP (KIF26B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 17422-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17422-1-AP (KIF26B Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 17422-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17422-1-AP (KIF26B Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MDCK-Zellen, HEK-293-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 9 publications below
• IHC: See 7 publications below
• IF: See 3 publications below

Produktinformation

17422-1-AP bindet in WB, IHC, IF, ELISA KIF26B und zeigt Reaktivität mit human, Hund

• Getestete Reaktivität: human, Hund
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KIF26B fusion protein Ag11446
• Vollständiger Name: kinesin family member 26B
• Berechnetes Molekulargewicht: 2108aa,224 kDa; 162aa,17 kDa
• Beobachtetes Molekulargewicht: 224-230 kDa
• GenBank-Zugangsnummer: BC042481
• Gene symbol: KIF26B
• Gene ID (NCBI): 55083
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KIF26B is a member of kinesin-11 family and plays important role in embryogenesis. It is a downstram target of Sall1 and is essential for kidney development. KIF26B is also involved in tumorigenesis.

Publikationen

Species	Application	Title
mouse,human	WB,IHC	Nat Commun; Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis.; Authors - Shujie Chen ; KD Validated
human	WB,IHC	Biomed Pharmacother; KIF26B promotes cell proliferation and migration through the FGF2/ERK signaling pathway in breast cancer.; Authors - Yan Teng
mouse,human	WB,IF	J Bone Miner Res; KIF26B Silencing Prevents Osseous Transdifferentiation of Progenitor/Stem Cells and Attenuates Ectopic Calcification in a Murine Model.; Authors - Mingming Yan ; KD Validated
human	IF	J Ginseng Res; Ginsenoside Rh2 reduces m6A RNA methylation in cancer via the KIF26B-SRF positive feedback loop; Authors - Chunmei Hu
human	IHC	Cell Cycle; Noncoding RNAs-based high KIF26B expression correlates with poor prognosis and tumor immune infiltration in colon cancer; Authors - Zhihong Liu
mouse	IF	Biochem Biophys Res Commun; Novel mechanistic role of Kif26b in adipogenic differentiation of murine multipotent stromal cells.; Authors - Mingming Yan ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Abigail (Verified Customer) (06-17-2024)

We had a lot of issues with this antibody due to difficulties transferring the high molecular weight protein. When we were able to get a good transfer as seen on the Ponceau, it was pretty hit or miss on if the antibody worked. We only had one quantifiable blot, the rest were not able to be transferred due to poor signal.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Brain cortex