KIF2A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

KIF2A Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(17)

Rezensionen (1)

Kat-Nr. : 13105-1-AP

Synonyme

HK2, KIF2, Kinesin 2, kinesin heavy chain member 2A, Kinesin like protein KIF2A

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  WB analysis of HeLa using 13105-1-AP

  WB result of KIF2A antibody (13105-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF2A transfected HeLa cells.

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  WB analysis using 13105-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13105-1-AP (KIF2A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13105-1-AP

  IP result of anti-KIF2A (IP:13105-1-AP, 4ug; Detection:13105-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human breast cancer using 13105-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13105-1-AP (KIF2A Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 13105-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13105-1-AP (KIF2A Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of MDCK using 13105-1-AP

  Immunofluorescent analysis of MDCK cells using 13105-1-AP (KIF2A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A549 using 13105-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using KIF2A antibody (13105-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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  FC experiment of A549 using 13105-1-AP

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human KIF2A (13105-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen, MDCK-Zellen
• Erfolgreiche Detektion in FC (Intra): A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IF: See 10 publications below
• IP: See 1 publications below

Produktinformation

13105-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA KIF2A und zeigt Reaktivität mit human, Hund, Maus, Ratten

• Getestete Reaktivität: human, Hund, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KIF2A fusion protein Ag3748
• Vollständiger Name: kinesin heavy chain member 2A
• Berechnetes Molekulargewicht: 679 aa, 77 kDa
• Beobachtetes Molekulargewicht: 80-100 kDa
• GenBank-Zugangsnummer: BC031828
• Gene symbol: KIF2A
• Gene ID (NCBI): 3796
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Kinesin family member 2a (KIF2A), one of the kinesin family members, is identified in mammalian cells and involved in regulating microtubule dynamics during the mitosis via modulating intracellular transport, cell division, and bipolar spindle assembly (PMID: 17538014). High expression of KIF2A correlates with advanced tumor stages and worse prognosis in non-small cell lung cancer patients (PMID: 31858647). Within clinical practice, KIF2A exhibits key prognostic value for the management of gastric cancer, colorectal cancer and nasopharyngeal carcinoma (PMID: 32106376, PMID: 26070867, PMID: 26602436).

Publikationen

Species	Application	Title
human	WB	EMBO J; The CEP170B-KIF2A complex destabilizes microtubule minus ends to generate polarized microtubule network; Authors - Cuirong Guan ; KD Validated
human	WB,IF	J Cell Biol; WDR62 regulates spindle dynamics as an adaptor protein between TPX2/Aurora A and katanin.; Authors - Junjie Huang
mouse	WB,IF	EMBO Rep; Mcrs1 regulates G2/M transition and spindle assembly during mouse oocyte meiosis; Authors - Jia-Qian Ju
human	WB	Front Oncol; Identification of Glycolysis-Related lncRNAs and the Novel lncRNA WAC-AS1 Promotes Glycolysis and Tumor Progression in Hepatocellular Carcinoma.; Authors - Xigang Xia
human	WB	Biomed Pharmacother; KIF2A promotes the progression via AKT signaling pathway and is upregulated by transcription factor ETV4 in human gastric cancer.; Authors - Xin Zhang ; KD Validated
human	IF	Mol Biol Cell; Mps1 regulates spindle morphology through MCRS1 to promote chromosome alignment.; Authors - Hongdan Yang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Dorota (Verified Customer) (04-10-2019)

Good for western blotting.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:250 -1:1000
• Cell Tissue Type: U2OS

KIF2A Antibody Western Blot, validation (1:250 -1:1000 dilution) in U2OS (Cat no:13105-1-AP)