KIF3A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

KIF3A Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 13930-1-AP

Synonyme

KIF3, KIF3A, kinesin family member 3A, Kinesin like protein KIF3A

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 13930-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13930-1-AP (KIF3A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human cerebellum using 13930-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 13930-1-AP (KIF3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 13930-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 13930-1-AP (KIF3A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13930-1-AP

  IP Result of anti-KIF3A (IP:13930-1-AP, 4ug; Detection:13930-1-AP 1:500) with mouse brain tissue lysate 8000ug.

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  IP experiment using 13930-1-AP

  WB and IP result of the antibdoy anti-KIF3A (13930-1-AP) in mouse cell from Corbit, Kevin.

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  IHC staining of human gliomas using 13930-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13930-1-AP (KIF3A antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human gliomas using 13930-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13930-1-AP (KIF3A antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Cerebellum-Gewebe, Maus-Cerebellum-Gewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 11 publications below
• IF: See 4 publications below

Produktinformation

13930-1-AP bindet in WB, IP, IHC, IF, ELISA KIF3A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KIF3A fusion protein Ag4954
• Vollständiger Name: kinesin family member 3A
• Berechnetes Molekulargewicht: 80 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC045542
• Gene symbol: KIF3A
• Gene ID (NCBI): 11127
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KIF3A is a subunit of Kinesin II that is essential for intraflagellar transport (IFT). It functions as the anterograde motor to deliver ciliary components to the tip where the axoneme is assembled. Disruption of KIF3A has been reported to block mammalian ciliogenesis. Recently KIF3A has been found to localize to the mother centriole and is required for centrioles organization. This antibody specifically recognizes the endogenous KIF3A protein in mouse embryonic fibroblasts (MEFs) but not in KIF3A-/- lysate. (23386061)

Publikationen

Species	Application	Title
human	WB	Nat Commun; A stress-induced cilium-to-PML-NB route drives senescence initiation; Authors - Xiaoyu Ma
human	WB	Sci Adv; An EMT-primary cilium-GLIS2 signaling axis regulates mammogenesis and claudin-low breast tumorigenesis.; Authors - Molly M Wilson
human		Nat Commun; Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.; Authors - Hai-Qing Tu
human	WB	Proc Natl Acad Sci U S A; EMT programs promote basal mammary stem cell and tumor-initiating cell stemness by inducing primary ciliogenesis and Hedgehog signaling.; Authors - Vincent J Guen ; KO Validated
mouse, human	WB,IF	EMBO J; Kif3a interacts with Dynactin subunit p150 Glued to organize centriole subdistal appendages.; Authors - Kodani Andrew A ; KD Validated
human	WB,IF	Cell Mol Life Sci; Requirement of NPHP5 in the hierarchical assembly of basal feet associated with basal bodies of primary cilia.; Authors - Delowar Hossain ; KD Validated