KIFC1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

KIFC1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Peptide array, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 20790-1-AP

Synonyme

HSET, KIFC1, kinesin family member C1, Kinesin like protein 2, Kinesin like protein KIFC1, Kinesin related protein HSET, KNSL2

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Galerie der Validierungsdaten

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  WB analysis of rat testis using 20790-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 20790-1-AP (KIFC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 20790-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20790-1-AP (KIFC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20790-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20790-1-AP (KIFC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 20790-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20790-1-AP (KIFC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 20790-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20790-1-AP (KIFC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 20790-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20790-1-AP (KIFC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 20790-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20790-1-AP (KIFC1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 20790-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20790-1-AP (KIFC1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 20790-1-AP

  Immunofluorescent analysis of Hela cells, using KIFC1 antibody 20790-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of HepG2 using 20790-1-AP

  Immunofluorescent analysis of HepG2 cells, using KIFC1 antibody 20790-1-AP at 1:25 dilution and FITC- labeled donkey anti-Rabbit IgG (green).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhodengewebe, HeLa-Zellen, Maushodengewebe, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

20790-1-AP bindet in WB, Peptide array, IHC, IF, ELISA KIFC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KIFC1 fusion protein Ag14492
• Vollständiger Name: kinesin family member C1
• Berechnetes Molekulargewicht: 673 aa, 74 kDa
• Beobachtetes Molekulargewicht: 55-70 kDa
• GenBank-Zugangsnummer: BC000712
• Gene symbol: KIFC1
• Gene ID (NCBI): 3833
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KIFC1 is a molecular motor protein belonging to the kinesin-14 family that has motor domains at the C-terminus. KIFC1 is widely distributed, with highest expression in testis and participating in acrosome biogenesis and vesicle transport. It is also abundant in ovary, spleen, and liver. KIFC1 proteins are generally localized in the perinuclear region, and have been found to be involved chromosome alignment, congression and intracellular DNA transport. This antibody was raised against the C-terminal region of human KIFC1 protein, and recognizes 55-70 kDa of endogenous KIFC1 protein in multiple tissue lysates. (12826589, 15236353, 23543461)

Publikationen

Species	Application	Title
mouse	WB,IF,Peptide array	Science; Mechanism of spindle pole organization and instability in human oocytes.; Authors - Chun So ; KD Validated
human	WB	Dev Cell; Direct Microtubule-Binding by Myosin-10 Orients Centrosomes toward Retraction Fibers and Subcortical Actin Clouds.; Authors - Mijung Kwon
human	WB	Cell Mol Life Sci; Proteomic interaction profiling reveals KIFC1 as a factor involved in early targeting of F508del-CFTR to degradation.; Authors - Sara Canato ; KD Validated
rat	WB	J Neurosci; KIFC1 regulates the trajectory of neuronal migration.; Authors - Hemalatha Muralidharan ; KD Validated
human	IF	FASEB J; Export of membrane proteins from the Golgi complex to the primary cilium requires the kinesin motor, KIFC1.; Authors - Si-Hyung Lee
human	IHC	Front Mol Biosci; Prognostic Value and Immunological Role of KIFC1 in Hepatocellular Carcinoma.; Authors - Dan Li