KIR2DS4/CD158i Polyklonaler Antikörper

KIR2DS4/CD158i Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21152-1-AP

Synonyme

KIR2DS4, CD158 antigen-like family member I, CD158I, Killer cell immunoglobulin-like receptor 2DS4, KIR1D

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  WB analysis of HL-60 using 21152-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 21152-1-AP (KIR2DS4/CD158i antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 21152-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21152-1-AP (KIR2DS4/CD158i antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 21152-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 21152-1-AP (KIR2DS4/CD158i antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 21152-1-AP

  Immunofluorescent analysis of HepG2 cells, using KIR2DS4/CD158i antibody 21152-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

21152-1-AP bindet in WB, IF/ICC, ELISA KIR2DS4/CD158i und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KIR2DS4/CD158i fusion protein Ag15711
• Vollständiger Name: killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 4
• Berechnetes Molekulargewicht: 304 aa, 34 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC096694
• Gene symbol: KIR2DS4
• Gene ID (NCBI): 3809
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KIR2DS4 (also known as CD158i) is a member of the killer cell immunoglobulin-like receptor (KIR) family. KIRs are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. KIRs are mainly involved in inhibiting NK killing (inhibitory KIRs) via interaction with MHC class I molecules. KIR2DS4 has been reported to be an activating KIR and recognize the HLA-Cw4 protein.