KIR3DL1 Polyklonaler Antikörper

KIR3DL1 Polyklonal Antikörper für FC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC,ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 13335-1-AP

Synonyme

CD158E, CD158E1, KIR, KIR3DL1, MHC class I NK cell receptor, NKAT 3, NKAT3, NKB1, NKB1B, p70 NK receptor CL 2/CL 11

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Galerie der Validierungsdaten

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  WB analysis of human liver using 13335-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13335-1-AP (KIR3DL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  FC experiment of Jurkat using 13335-1-AP

  1X10^6 Jurkat cells were stained with 0.2ug KIR3DL1 antibody (13335-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lebergewebe
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

13335-1-AP bindet in WB, FC,ELISA KIR3DL1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KIR3DL1 fusion protein Ag4152
• Vollständiger Name: killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail, 1
• Berechnetes Molekulargewicht: 444 aa, 49 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC028206
• Gene symbol: KIR3DL1
• Gene ID (NCBI): 3811
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR3DL1 is a receptor on natural killer (NK) cells for HLA Bw4 allele. It inhibits the activity of NK cells thus preventing cell lysis. KIR3DL1 and KIR3DS1 are highly homologous. This antibody is raised against 22-344 amino acids of human KIR3DL1 and recognizes both KIR3DL1 (49 kDa) and KIR3DS1 (43 kDa).

Publikationen

Species	Application	Title
		Clin Exp Immunol; Autoantibodies to Killer Cell Immunoglobulin-Like Receptor 3DL1 in Patients with Systemic Lupus Erythematosus.; Authors - Akihiro Doi