c-Kit/CD117 Polyklonaler Antikörper

c-Kit/CD117 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

22

Kat-Nr. : 18696-1-AP

Synonyme

C Kit, CD117, c-Kit, c-Kit/CD117, KIT, PBT, Proto oncogene c Kit, SCFR, Tyrosine protein kinase Kit

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Galerie der Validierungsdaten

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  WB analysis of Mo7e using 18696-1-AP

  Mo7e cells were subjected to SDS PAGE followed by western blot with 18696-1-AP (c-Kit/CD117 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of stromal tumor using 18696-1-AP

  Immunohistochemical analysis of paraffin-embedded stromal tumor tissue slide using 18696-1-AP (c-Kit/CD117 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of stromal tumor using 18696-1-AP

  Immunohistochemical analysis of paraffin-embedded stromal tumor tissue slide using 18696-1-AP (c-Kit/CD117 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of Insulinoma using 18696-1-AP

  Immunohistochemical analysis of paraffin-embedded Insulinoma tissue slide using 18696-1-AP (c-Kit/CD117 antibody) at dilution of 1:2000 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of Insulinoma using 18696-1-AP

  Immunohistochemical analysis of paraffin-embedded Insulinoma tissue slide using 18696-1-AP (c-Kit/CD117 antibody) at dilution of 1:2000 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IHC staining of human placenta using 18696-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18696-1-AP (KIT antibody) at dilution of 1:400 (under 40x lens).

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  IHC staining of human testis using 18696-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18696-1-AP (KIT antibody) at dilution of 1:400 (under 40x lens).

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  IF Staining of MCF-7 using 18696-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 18696-1-AP (c-Kit/CD117 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of MCF-7 using 18696-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug c-Kit/CD117 antibody (18696-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mo7e cells
• Erfolgreiche Detektion in IHC: Stromatumorgewebe, humanes Hodengewebe, humanes Plazenta-Gewebe, Insulinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 8 publications below
• IF: See 7 publications below

Produktinformation

18696-1-AP bindet in WB, IHC, IF, FC, ELISA c-Kit/CD117 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• Berechnetes Molekulargewicht: 110 kDa
• Beobachtetes Molekulargewicht: 140-160 kDa
• GenBank-Zugangsnummer: BC071593
• Gene symbol: KIT
• Gene ID (NCBI): 3815
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KIT, also named as SCFR, c-Kit and CD117, is a transmembrane tyrosine kinase encoded by the c-Kit proto oncogene. It is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand(SCF) binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. Phosphorylation at tyrosine 721 of KIT allows binding and activation of PI3 kinase. Loss of expression of KIT appears to be associated with progression of some tumors (melanoma) and autocrine/paracrine stimulation of the kit/SCF system may participate in human solid tumors such as lung, breast, testicular and gynecological malignancies. Mutations in Kit have been found to be important for tumor growth and progression in a variety of cancers including mast cell diseases, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma and lung cancer.

Publikationen

Species	Application	Title
human	WB	Mech Ageing Dev; Acceleration of melanocyte senescence by the proinflammatory cytokines IFNγ and TNFα impairs the repigmentation response of vitiligo patients to narrowband ultraviolet B (NBUVB) phototherapy; Authors - Bing-Qi Dong
human	IHC	Front Cell Dev Biol; Single-Cell Sequencing Unveils the Heterogeneity of Nonimmune Cells in Chronic Apical Periodontitis.; Authors - Xinwei Lin
human	IHC	Front Immunol; Immune Infiltrating Cells-Derived Risk Signature Based on Large-scale Analysis Defines Immune Landscape and Predicts Immunotherapy Responses in Glioma Tumor Microenvironment.; Authors - Nan Zhang
human	IF	J Exp Clin Cancer Res; Growth differentiation factor 15 contributes to marrow adipocyte remodeling in response to the growth of leukemic cells.; Authors - Wei Lu
mouse	IF	Andrology; Exposure to elevated temperature affects the expression of piwi-interacting RNAs and associated transcripts in mouse testes; Authors - Wenbin Chen
human	WB	Tumour Biol; Orai1 mediates tumor-promoting store-operated Ca(2+) entry in human gastrointestinal stromal tumors via c-KIT and the extracellular signal-regulated kinase pathway.; Authors - Lei Wang

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