CD117/c-Kit Polyklonaler Antikörper

CD117/c-Kit Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(37)

Rezensionen (1)

Kat-Nr. : 18696-1-AP

Synonyme

CD117, KIT, Mast/stem cell growth factor receptor Kit, EC:2.7.10.1, c-Kit

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  WB analysis of Mo7e using 18696-1-AP

  Mo7e cells were subjected to SDS PAGE followed by western blot with 18696-1-AP (CD117/c-kit antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of stromal tumor using 18696-1-AP

  Immunohistochemical analysis of paraffin-embedded stromal tumor tissue slide using 18696-1-AP (CD117/c-kit antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of stromal tumor using 18696-1-AP

  Immunohistochemical analysis of paraffin-embedded stromal tumor tissue slide using 18696-1-AP (CD117/c-kit antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human placenta using 18696-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18696-1-AP (CD117/c-kit antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human testis using 18696-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18696-1-AP (CD117/c-kit antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of Insulinoma using 18696-1-AP

  Immunohistochemical analysis of paraffin-embedded Insulinoma tissue slide using 18696-1-AP (CD117/c-kit antibody) at dilution of 1:2000 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of Insulinoma using 18696-1-AP

  Immunohistochemical analysis of paraffin-embedded Insulinoma tissue slide using 18696-1-AP (CD117/c-kit antibody) at dilution of 1:2000 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IF Staining of MCF-7 using 18696-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 18696-1-AP (CD117/c-kit antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mo7e cells
• Erfolgreiche Detektion in IHC: Stromatumorgewebe, humanes Plazenta-Gewebe, humanes Hodengewebe, Insulinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 14 publications below
• IHC: See 14 publications below
• IF: See 11 publications below

Produktinformation

18696-1-AP bindet in WB, IHC, IF/ICC, ELISA CD117/c-Kit und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• Berechnetes Molekulargewicht: 110 kDa
• Beobachtetes Molekulargewicht: 140-160 kDa
• GenBank-Zugangsnummer: BC071593
• Gene symbol: CD117
• Gene ID (NCBI): 3815
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early hemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).

Publikationen

Species	Application	Title
human	WB	Adv Healthc Mater; ROS-Responsive Hydrogel Delivering METRNL Enhances Bone Regeneration via Dual Stem Cell Homing and Vasculogenesis Activation; Authors - Yue Xu
human	WB	Mech Ageing Dev; Acceleration of melanocyte senescence by the proinflammatory cytokines IFNγ and TNFα impairs the repigmentation response of vitiligo patients to narrowband ultraviolet B (NBUVB) phototherapy; Authors - Bing-Qi Dong
human	IHC	Front Cell Dev Biol; Single-Cell Sequencing Unveils the Heterogeneity of Nonimmune Cells in Chronic Apical Periodontitis.; Authors - Xinwei Lin
human	IHC	Front Immunol; Immune Infiltrating Cells-Derived Risk Signature Based on Large-scale Analysis Defines Immune Landscape and Predicts Immunotherapy Responses in Glioma Tumor Microenvironment.; Authors - Nan Zhang
human	IF	J Exp Clin Cancer Res; Growth differentiation factor 15 contributes to marrow adipocyte remodeling in response to the growth of leukemic cells.; Authors - Wei Lu
mouse	IF	Andrology; Exposure to elevated temperature affects the expression of piwi-interacting RNAs and associated transcripts in mouse testes; Authors - Wenbin Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (02-14-2025)

Interestingly, CD117 (in green), marker of stem cells, seem to have marked some of the neurons (in red) of my epileptic TSC FFPE human cortex tissue.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

CD117/c-Kit Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:18696-1-AP)