KLF15 Monoklonaler Antikörper

KLF15 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgM

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

2B8G9

Kat-Nr. : 66185-1-Ig

Synonyme

KKLF, KLF15, Krueppel like factor 15, Kruppel like factor 15

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Galerie der Validierungsdaten

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  WB analysis of human skeletal muscle using 66185-1-Ig

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66185-1-Ig (KLF15 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66185-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66185-1-Ig (KLF15 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Skelettmuskelgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

66185-1-Ig bindet in WB, ELISA KLF15 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgM
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: KLF15 fusion protein Ag4864
• Vollständiger Name: Kruppel-like factor 15
• Berechnetes Molekulargewicht: 416 aa, 44 kDa
• Beobachtetes Molekulargewicht: 44-55 kDa
• GenBank-Zugangsnummer: BC036733
• Gene symbol: KLF15
• Gene ID (NCBI): 28999
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KLF15, a kidney-enriched Krüppel-like zinc finger-containing transcription factors, is a transcriptional activator that binds the CLCNKA promoter. The nuclear protein KLF15, is expressed primarily in liver, heart, skeletal muscle and kidney tissues but is not detected in lymphoid tissues or bone marrow. It is an important regulator of GLUT4 in both adipose and muscle tissues.(PMID:10982849,PMID:16615998).

Publikationen

Species	Application	Title
human	WB	Nucleic Acids Res; EWS-FLI1 regulates and cooperates with core regulatory circuitry in Ewing sarcoma.; Authors - Xianping Shi
mouse	WB	Front Cell Dev Biol; Krüppel-Like Factor 15 Modulates CXCL1/CXCR2 Signaling-Mediated Inflammatory Response Contributing to Angiotensin II-Induced Cardiac Remodeling.; Authors - Shun He ; KO Validated
mouse	WB	Cell Rep; The Mediator subunit MED20 organizes the early adipogenic complex to promote development of adipose tissues and diet-induced obesity.; Authors - Wen-Shuai Tang