KLF4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

KLF4 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

112

Kat-Nr. : 11880-1-AP

Synonyme

EZF, GKLF, KLF4, Krueppel like factor 4, Kruppel like factor 4 (gut)

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Galerie der Validierungsdaten

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  WB analysis using 11880-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11880-1-AP (KLF4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11880-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11880-1-AP (KLF4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11880-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11880-1-AP (KLF4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11880-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11880-1-AP (KLF4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11880-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11880-1-AP (KLF4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 11880-1-AP

  NCCIT cell were subjected to SDS PAGE followed by western blot with 11880-1-AP (KLF4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11880-1-AP

  IP result of anti-KLF4(IP:11880-1-AP, 4ug; Detection:11880-1-AP 1:8000) with HeLa cells lysate 1320 ug.

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  IHC staining of mouse embryo using 11880-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 11880-1-AP (KLF4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 11880-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 11880-1-AP (KLF4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 11880-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 11880-1-AP (KLF4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 11880-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 11880-1-AP (KLF4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsil using 11880-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil using 11880-1-AP (KLF4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsil using 11880-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil using 11880-1-AP (KLF4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 11880-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 11880-1-AP (KLF4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 11880-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 11880-1-AP (KLF4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of human embronic stem cells using 11880-1-AP

  Confocal immunofluorescent analysis of human embronic stem cells with 11880-1-AP at dilution of 1:50. The PE shows staining with 11880-1-AP/PE. The DAPI shows nuclear staining by DAPI.

• 

  FC experiment of HeLa using 11880-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human KLF4 (11880-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, A549-Zellen, C2C12-Zellen, HEK-293-Zellen, HeLa-Zellen, HT-1080.Zellen, HT-29-Zellen, HUVEC-Zellen, Mauslebergewebe, Mauslungengewebe, NCCIT-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Mausembryogewebe, humanes Kolongewebe, humanes Milzgewebe, humanes Tonsillengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humane embryonale Stammzellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 15 publications below
• WB: See 89 publications below
• IHC: See 16 publications below
• IF: See 23 publications below
• IP: See 4 publications below
• FC: See 1 publications below
• ChIP: See 8 publications below

Produktinformation

11880-1-AP bindet in WB, IP, IHC, IF, FC, ChIP, ELISA KLF4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KLF4 fusion protein Ag2480
• Vollständiger Name: Kruppel-like factor 4 (gut)
• Berechnetes Molekulargewicht: 504 aa, 54 kDa
• Beobachtetes Molekulargewicht: 50-60 kDa
• GenBank-Zugangsnummer: BC030811
• Gene symbol: KLF4
• Gene ID (NCBI): 9314
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The mammalian Krüppel-like transcription factor 4 (Klf4) is an evolutionarily conserved zinc finger-containing transcription factor with diverse regulatory functions in cell growth, proliferation, differentiation and embryogenesis. It also plays a significant role in the process of tumorigenesis. Klf4 is one of the key transcription factors that have been used to reprogram mouse and human fibroblasts to a pluripotent state also called induced pluripotent stem (iPS) cells. Affinity purified rabbit anti- Klf4 is for the detection of Klf4 transgene expression in the process of reprogramming. There are two isoforms of KLF4 (PMID: 15561714), with MW range from 50 to 60 kDa,and major band is 55 kDa. KLF4 protein can be ubiquitin modified.

Publikationen

Species	Application	Title
mouse	WB,IF	Nat Commun; Klf4 glutamylation is required for cell reprogramming and early embryonic development in mice.; Authors - Buqing Ye
human	WB,IHC	Nat Commun; LncBRM initiates YAP1 signalling activation to drive self-renewal of liver cancer stem cells.; Authors - Pingping Zhu
human	WB,IHC	J Hematol Oncol; A novel lncRNA ROPM-mediated lipid metabolism governs breast cancer stem cell properties.; Authors - Shuiqing Liu
human	IF	Mater Today Bio; Cellular reprogramming with multigene activation by the delivery of CRISPR/dCas9 ribonucleoproteins via magnetic peptide-imprinted chitosan nanoparticles.; Authors - Mei-Hwa Lee
mouse	WB	EMBO J; Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.; Authors - Lihua Wang
mouse	IF,WB	J Neuroinflammation; The critical role of KLF4 in regulating the activation of A1/A2 reactive astrocytes following ischemic stroke; Authors - Cong Wang

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