KLF5 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

KLF5 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

40

Rezensionen (1)

Kat-Nr. : 21017-1-AP

Synonyme

BTE binding protein 2, BTEB2, CKLF, Colon krueppel like factor, GC box binding protein 2, IKLF, KLF5, Krueppel like factor 5, Transcription factor BTEB2

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Galerie der Validierungsdaten

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  WB analysis using 21017-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21017-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21017-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21017-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 21017-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 21017-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 21017-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 21017-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 21017-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 21017-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 21017-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 21017-1-AP

  IP Result of anti-KLF5 (IP:21017-1-AP, 4ug; Detection:21017-1-AP 1:500) with HeLa cells lysate 2800ug.

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  IHC staining of mouse embryo using 21017-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 21017-1-AP (KLF5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 21017-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21017-1-AP (KLF5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 21017-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21017-1-AP (KLF5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 21017-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 21017-1-AP (KLF5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 21017-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 21017-1-AP (KLF5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Caco-2-Zellen, COLO 320-Zellen, HCT 116-Zellen, HeLa-Zellen, HT-1080.Zellen, humanes Hodengewebe, Maus-Dünndarmgewebe, Maus-Kolongewebe, PC-3-Zellen, Ratten-Kolongewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, Mausembryogewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 12 publications below
• WB: See 31 publications below
• IHC: See 9 publications below
• IF: See 7 publications below
• IP: See 1 publications below
• CoIP: See 3 publications below
• ChIP: See 4 publications below

Produktinformation

21017-1-AP bindet in WB, IP, IHC, IF, CoIP, ChIP, ELISA KLF5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KLF5 fusion protein Ag15267
• Vollständiger Name: Kruppel-like factor 5 (intestinal)
• Berechnetes Molekulargewicht: 457 aa, 51 kDa
• Beobachtetes Molekulargewicht: 51-55 kDa, 85-120 kDa
• GenBank-Zugangsnummer: BC042131
• Gene symbol: KLF5
• Gene ID (NCBI): 688
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling. This antibody detects the 50-60 kDa isoform 1 of KLF5. The antibody can recognize isoform 2 (33 kDa) and isoform 3 (18 kDa) too. 85 kDa, 95 kDa and 120 kDa of SUMOylated KLF5 were detected (PMID: 18500350).

Publikationen

Species	Application	Title
human	WB	Cell Res; ERK kinase phosphorylates and destabilizes the tumor suppressor FBW7 in pancreatic cancer.; Authors - Shunrong Ji
human	WB	Cell Death Dis; DNMT1-maintained hypermethylation of Krüppel-like factor 5 involves in the progression of clear cell renal cell carcinoma.; Authors - Rong-Jie Fu ; KD Validated
human	WB,IHC	Aging (Albany NY); Overexpression of KLF5 is associated with poor survival and G1/S progression in pancreatic cancer.; Authors - Yilong Li ; KD Validated
human	WB	Oncotarget; Ascl2 activation by YAP1/KLF5 ensures the self-renewability of colon cancer progenitor cells.; Authors - Xiaolong Wei ; KD Validated
mouse	WB	Eur J Pharmacol; Early growth response 1/Krüppel-like factor 5 pathway inhibitor alleviates lipopolysaccharide-induced lung injury by promoting autophagy; Authors - Kang Zou
human	WB	Front Oncol; A Weighted Gene Co-Expression Network Analysis-Derived Prognostic Model for Predicting Prognosis and Immune Infiltration in Gastric Cancer.; Authors - Qingchuan Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sarah-Eve (Verified Customer) (03-01-2023)

Works okay on pvdf membrane, blocking with skim milk. A lot of bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Human pulmonary smooth muscle cells

KLF5 Antibody Western Blot validation (1:2000 dilution) in Human pulmonary smooth muscle cells (Cat no:21017-1-AP)