KLHL14 Polyklonaler Antikörper

KLHL14 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16693-1-AP

Synonyme

kelch like 14 (Drosophila), Kelch like protein 14, KIAA1384, KLHL14, Printor, Protein interactor of Torsin-1A

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Galerie der Validierungsdaten

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  WB analysis of PC-3 using 16693-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16693-1-AP (KLHL14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16693-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16693-1-AP (KLHL14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16693-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16693-1-AP (KLHL14 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 16693-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16693-1-AP (KLHL14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 16693-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16693-1-AP (KLHL14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 16693-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16693-1-AP (KLHL14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, HepG2-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16693-1-AP bindet in WB, IF, ELISA KLHL14 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KLHL14 fusion protein Ag10065
• Vollständiger Name: kelch-like 14 (Drosophila)
• Berechnetes Molekulargewicht: 44 kDa, 71 kDa
• Beobachtetes Molekulargewicht: 35 kDa, 70 kDa
• GenBank-Zugangsnummer: BC021267
• Gene symbol: KLHL14
• Gene ID (NCBI): 57565
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Kelch-like protein 14 (KLHL14) is a member of the KLHL family. The kelch motif was initially discovered in Kelch. In this protein there are six copies of the motif. It has been shown for one member that it is related to Galactose Oxidase for which a structure has been solved. Two isoforms of this protein exist - 70kDa and 43kDa.