KLHL2 Polyklonaler Antikörper

KLHL2 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14449-1-AP

Synonyme

Kelch-like protein 2, Actin-binding protein Mayven, ABP KELCH

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  WB analysis of MCF-7 using 14449-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14449-1-AP (KLHL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 14449-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14449-1-AP (KLHL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14449-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14449-1-AP (KLHL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 14449-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using KLHL2 antibody (14449-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, humanes Hirngewebe, MCF-7-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

14449-1-AP bindet in WB, IF/ICC, ELISA KLHL2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KLHL2 fusion protein Ag5950
• Vollständiger Name: kelch-like 2, Mayven (Drosophila)
• Berechnetes Molekulargewicht: 66 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: BC022503
• Gene symbol: KLHL2
• Gene ID (NCBI): 11275
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KLHL2 contains an SH3-binding domain in the N terminus and a POZ domain and 6 tandem repeats of approximately 50 amino acids each in the C terminus. In astrocytoma/glioblastoma cells, KLHL2 colocalized with actin filaments in the cytoskeleton remodeling regions in stress fibers and in patchy cortical actin-rich regions of the cell margins. In primary rat hippocampal neurons, it was highly expressed in the cell body and in neurite processes(PMID: 10397770).