KLHL31 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

KLHL31 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24781-1-AP

Synonyme

BKLHD6, KBTBD1, kelch like 31 (Drosophila), Kelch like protein 31, Kelch like protein KLHL, KLHL, KLHL31

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  WB analysis using 24781-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24781-1-AP (KLHL31 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse embryo using 24781-1-AP

  mouse embryo tissue were subjected to SDS PAGE followed by western blot with 24781-1-AP (KLHL31 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 24781-1-AP

  WB result of KLHL31 antibody (24781-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KLHL31 transfected Jurkat cells.

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  IHC staining of mouse skeletal muscle using 24781-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 24781-1-AP (KLHL31 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 24781-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 24781-1-AP (KLHL31 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 24781-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24781-1-AP (KLHL31 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 24781-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24781-1-AP (KLHL31 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 24781-1-AP

  Immunofluorescent analysis of HepG2 cells using 24781-1-AP (KLHL31 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausembryogewebe, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

24781-1-AP bindet in WB, IHC, IF/ICC, ELISA KLHL31 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KLHL31 fusion protein Ag20462
• Vollständiger Name: kelch-like 31 (Drosophila)
• Berechnetes Molekulargewicht: 634 aa, 70 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC137267
• Gene symbol: KLHL31
• Gene ID (NCBI): 401265
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KLHL31 also named as BKLHD6, KBTBD1,or Kelch like protein 31 is a 634 amino acid protein, which contains one 1 BACK domain, one BTB domain and six Kelch repeats. KLHL31 as a transcription repressor in MAPK/JNK signaling pathway to regulate cellular functions. KLHL31 is strongly expressed in skeletal muscle and weakly in heart.