KLK8 Monoklonaler Antikörper

KLK8 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2F3A8

Kat-Nr. : 68247-1-Ig

Synonyme

Kallikrein 8, Kallikrein-8, Kallikrein8, kallikrein related peptidase 8, EC:3.4.21.118

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  WB analysis using 68247-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68247-1-Ig (KLK8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of EC109 using 68247-1-Ig

  EC109 cells were subjected to SDS PAGE followed by western blot with 68247-1-Ig (KLK8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 68247-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 68247-1-Ig (KLK8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 68247-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68247-1-Ig (KLK8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 68247-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68247-1-Ig (KLK8 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 68247-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68247-1-Ig (KLK8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 68247-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68247-1-Ig (KLK8 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 68247-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using KLK8 antibody (68247-1-Ig, Clone: 2F3A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of SH-SY5Y using 68247-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using KLK8 antibody (68247-1-Ig, Clone: 2F3A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, A431-Zellen, EC109-Zellen, MOLT-4-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68247-1-Ig bindet in WB, IHC, IF/ICC, ELISA KLK8 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: KLK8 fusion protein Ag5642
• Vollständiger Name: kallikrein-related peptidase 8
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC040887
• Gene symbol: KLK8
• Gene ID (NCBI): 11202
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KLK8(Kallikrein-8) is also named as NRPN, PRSS19, TADG14.It is a new member of the human kallikrein family, which is predicted to be secreted and expected to be present in biological fluids (PMID:12507964).The protein is detectable in ovarian cancer tissue extracts, serum, and ascites fluid, indicating that it may serve as a new ovarian cancer marker (PMID:12782581). KLK8 has one predicted glycosylation site (PMID:20940292) and it has some isoforms produced by alternative splicing.