KLRB1/CD161 Monoklonaler Antikörper
KLRB1/CD161 Monoklonal Antikörper für WB, IHC, ELISA
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
human
Anwendung
WB, IHC, IF, ELISA
Konjugation
Unkonjugiert
CloneNo.
3D8F5
Kat-Nr. : 67537-1-Ig
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | humanes Milzgewebe |
| Erfolgreiche Detektion in IHC | humanes Tonsillitisgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunhistochemie (IHC) | IHC : 1:2000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 2 publications below |
| IHC | See 4 publications below |
| IF | See 2 publications below |
Produktinformation
67537-1-Ig bindet in WB, IHC, IF, ELISA KLRB1/CD161 und zeigt Reaktivität mit human
| Getestete Reaktivität | human |
| In Publikationen genannte Reaktivität | human |
| Wirt / Isotyp | Maus / IgG1 |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | KLRB1/CD161 fusion protein Ag27117 |
| Vollständiger Name | killer cell lectin-like receptor subfamily B, member 1 |
| Berechnetes Molekulargewicht | 225 aa, 25 kDa |
| Beobachtetes Molekulargewicht | 32 kDa |
| GenBank-Zugangsnummer | BC114516 |
| Gene symbol | CD161 |
| Gene ID (NCBI) | 3820 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein-G-Reinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
CD161, also known as KLRB1 or NKR-P1A, is a type II transmembrane C-type lectin-like receptor and is expressed on the cell membrane as disulfide-linked homodimer (PMID: 8077657). It is expressed by the majority of NK cells and subsets of peripheral T cells, including both CD4+ and CD8+ T cells, and is expressed preferentially on adult T cells with a "memory" antigenic phenotype (PMID: 8077657; 22566826). Expression of CD161 correlates with the cytotoxic function of CD16+ NK cells, and ligation of CD161 with its ligand LLT1 inhibits NK cell cytotoxicity and cytokine secretion (PMID: 29686665).
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for KLRB1/CD161 antibody 67537-1-Ig | Protokoll herunterladen |
| IHC protocol for KLRB1/CD161 antibody 67537-1-Ig | Protokoll herunterladenl |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Cell Rep Med Distinctive multicellular immunosuppressive hubs confer different intervention strategies for left- and right-sided colon cancers | ||
Front Immunol Comprehensive single-cell and bulk transcriptomic analyses to develop an NK cell-derived gene signature for prognostic assessment and precision medicine in breast cancer | ||
J Thorac Dis KLRB1 expression is associated with lung adenocarcinoma prognosis and immune infiltration and regulates lung adenocarcinoma cell proliferation and metastasis through the MAPK/ERK pathway | ||
Front Endocrinol (Lausanne) Machine learning immune-related gene based on KLRB1 model for predicting the prognosis and immune cell infiltration of breast cancer | ||
BMJ Open Respir Res Single-cell RNA-sequencing uncovers the dynamic changes of tumour immune microenvironment in advanced lung adenocarcinoma | ||
Front Immunol Single-cell sequencing analysis and multiple machine-learning models revealed the cellular crosstalk of dendritic cells and identified FABP5 and KLRB1 as novel biomarkers for psoriasis |



