KLRB1/CD161 Monoklonaler Antikörper

KLRB1/CD161 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

CloneNo.

3D8F5

Kat-Nr. : 67537-1-Ig

Synonyme

CD161, 3D8F5, CLEC5B, C-type lectin domain family 5 member B, hNKR P1A

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  WB analysis of human spleen using 67537-1-Ig

  human spleen tissue were subjected to SDS PAGE followed by western blot with 67537-1-Ig (KLRB1/CD161 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 67537-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67537-1-Ig (KLRB1/CD161 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 67537-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67537-1-Ig (KLRB1/CD161 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 67537-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67537-1-Ig (KLRB1/CD161 antibody) at dilution of 1:4000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Milzgewebe
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Produktinformation

67537-1-Ig bindet in WB, IHC, IF, ELISA KLRB1/CD161 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: KLRB1/CD161 fusion protein Ag27117
• Vollständiger Name: killer cell lectin-like receptor subfamily B, member 1
• Berechnetes Molekulargewicht: 225 aa, 25 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC114516
• Gene symbol: CD161
• Gene ID (NCBI): 3820
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD161, also known as KLRB1 or NKR-P1A, is a type II transmembrane C-type lectin-like receptor and is expressed on the cell membrane as disulfide-linked homodimer (PMID: 8077657). It is expressed by the majority of NK cells and subsets of peripheral T cells, including both CD4+ and CD8+ T cells, and is expressed preferentially on adult T cells with a "memory" antigenic phenotype (PMID: 8077657; 22566826). Expression of CD161 correlates with the cytotoxic function of CD16+ NK cells, and ligation of CD161 with its ligand LLT1 inhibits NK cell cytotoxicity and cytokine secretion (PMID: 29686665).

Publikationen

Species	Application	Title
human	IF	Cell Rep Med; Distinctive multicellular immunosuppressive hubs confer different intervention strategies for left- and right-sided colon cancers; Authors - Bingxin Liu
human	IHC	Front Immunol; Comprehensive single-cell and bulk transcriptomic analyses to develop an NK cell-derived gene signature for prognostic assessment and precision medicine in breast cancer; Authors - Qianshan Hou
human	WB	J Thorac Dis; KLRB1 expression is associated with lung adenocarcinoma prognosis and immune infiltration and regulates lung adenocarcinoma cell proliferation and metastasis through the MAPK/ERK pathway; Authors - Siwei Xu
human	WB	Front Endocrinol (Lausanne); Machine learning immune-related gene based on KLRB1 model for predicting the prognosis and immune cell infiltration of breast cancer; Authors - Guo Huang
human	IHC	BMJ Open Respir Res; Single-cell RNA-sequencing uncovers the dynamic changes of tumour immune microenvironment in advanced lung adenocarcinoma; Authors - Haijiao Lu
human	IHC	Front Immunol; Single-cell sequencing analysis and multiple machine-learning models revealed the cellular crosstalk of dendritic cells and identified FABP5 and KLRB1 as novel biomarkers for psoriasis; Authors - Zhiqiang Ma