KMO Monoklonaler Antikörper

KMO Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

CloneNo.

2D2F2

Rezensionen (1)

Kat-Nr. : 60029-1-Ig

Synonyme

2D2F2, EC:1.14.13.9, Kynurenine 3 hydroxylase, Kynurenine 3 monooxygenase, Kynurenine 3-hydroxylase

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• 

  WB analysis using 60029-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60029-1-Ig (KMO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, SKOV-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

60029-1-Ig bindet in WB, IHC, IF, ELISA KMO und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: KMO fusion protein Ag1109
• Vollständiger Name: kynurenine 3-monooxygenase (kynurenine 3-hydroxylase)
• Berechnetes Molekulargewicht: 486 aa, 56 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC005297
• Gene symbol: KMO
• Gene ID (NCBI): 8564
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KMO(Kynurenine 3-monooxygenase) is a membrane protein located on the outer membrane of mitochondria. Tissue distribution studies have revealed that, in rats, highest enzyme activity is found in kidney and liver, with brain having the least activity in comparison to peripheral organs(PMID: 9237672).

Publikationen

Species	Application	Title
mouse,human	IF	J Am Soc Nephrol; Loss of Kynurenine 3-Mono-oxygenase Causes Proteinuria.; Authors - Ron Korstanje
human	IHC	Neuro Oncol; Molecular imaging correlates of tryptophan metabolism via the kynurenine pathway in human meningiomas.; Authors - Edit Bosnyák
human	IHC	Oncol Lett; Viability of diffuse large B-cell lymphoma cells is regulated by kynurenine 3-monooxygenase activity; Authors - NANAKA MORITA
mouse	WB	Hum Mol Genet; Retention of stress susceptibility in the mdx mouse model of Duchenne muscular dystrophy after PGC-1α overexpression or ablation of IDO1 or CD38; Authors - Erynn E Johnson
mouse	IHC	Br J Pharmacol; Chronic stress induces behavioural changes through increased kynurenic acid by downregulation of kynurenine-3-monooxygenase with microglial decline; Authors - Masaya Hasegawa
rat	WB	J Pharmacol Sci; Thrombin-induced kynurenine 3-monooxygenase causes variations in the kynurenine pathway, leading to neurological deficits in a murine intracerebral hemorrhage model; Authors - Masatoshi Ohnishi

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hala (Verified Customer) (06-07-2022)

Works very good

• Applications: Western Blot
• Primary Antibody Dilution: 1/2000
• Cell Tissue Type: Huh6 and Hepg2