KMT2C Polyklonaler Antikörper

KMT2C Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 28437-1-AP

Synonyme

MLL3, Lysine N methyltransferase 2C, KIAA1506, Homologous to ALR protein, HALR

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  WB analysis using 28437-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 28437-1-AP (KMT2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 28437-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 28437-1-AP (KMT2C antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 28437-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 28437-1-AP (KMT2C antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

28437-1-AP bindet in WB, IHC, IF, CoIP, ChIP, ELISA KMT2C und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KMT2C fusion protein Ag28875
• Vollständiger Name: myeloid/lymphoid or mixed-lineage leukemia 3
• Berechnetes Molekulargewicht: 541 kDa
• Beobachtetes Molekulargewicht: 541kDa, 269 kDa
• GenBank-Zugangsnummer: NM_170606
• Gene symbol: KMT2C
• Gene ID (NCBI): 58508
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lysine (K)-specific methyltransferase 2C (KMT2C, also known as MLL3) belongs to the mixed-lineage leukemia (MLL) family of histone methyltransferases which methylate the histone 3 tail at lysine 4 (H3K4) as part of the complex proteins associated with Set 1 (COMPASS) complex. Although originally identified as oncogenic fusions in leukemia, genome-wide mutation studies have revealed frequent, presumably loss-of-function, mutations in various members of the MLL family, including MLL2/KMT2B, MLL3/KMT2C, and MLL4/KMT2D in a variety of malignancies, particularly solid tumors. Mechanistic studies of KMT2C in normal cells have focused primarily on its role in enhancer regulation by deposition of H3K4me1 marks.

Publikationen

Species	Application	Title
human	WB	Int Immunopharmacol; Polysaccharides from Platycodonis Radix ameliorated respiratory syncytial virus-induced epithelial cell apoptosis and inflammation through activation of miR-181a-mediated Hippo and SIRT1 pathways.; Authors - Juan-Juan Li
human	ChIP	J Biol Chem; TRIM28 represses renal cell carcinoma cell proliferation by inhibiting TFE3/KDM6A-regulated autophagy; Authors - Tanjing Song
human	WB,IF,CoIP	Adv Sci (Weinh); TAOK3 Facilitates Esophageal Squamous Cell Carcinoma Progression and Cisplatin Resistance Through Augmenting Autophagy Mediated by IRGM; Authors - Mingchuang Sun
human	WB	Nat Chem Biol; UFL1 triggers replication fork degradation by MRE11 in BRCA1/2-deficient cells; Authors - Tian Tian
Human		BMC Med Genomics; Clinical characteristics and genetic analysis of four pediatric patients with Kleefstra syndrome; Authors - Rong Ren