KPNA6 Polyklonaler Antikörper

KPNA6 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 12366-2-AP

Synonyme

Importin subunit alpha 7, IPOA7, Karyopherin subunit alpha 6, KPNA6

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  WB analysis of BxPC-3 using 12366-2-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12366-2-AP (KPNA6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12366-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12366-2-AP (KPNA6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 12366-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12366-2-AP (KPNA6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12366-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12366-2-AP (KPNA6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12366-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12366-2-AP (KPNA6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12366-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12366-2-AP (KPNA6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12366-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12366-2-AP (KPNA6 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

12366-2-AP bindet in WB, IHC, ELISA KPNA6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KPNA6 fusion protein Ag3040
• Vollständiger Name: karyopherin alpha 6 (importin alpha 7)
• Berechnetes Molekulargewicht: 536 aa, 60 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC020520
• Gene symbol: KPNA6
• Gene ID (NCBI): 23633
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Nuclear-capture of endosomes depletes nuclear G-actin to promote SRF/MRTF activation and cancer cell invasion.; Authors - Sergi Marco
human	WB	Virol Sin; African Swine Fever Virus MGF360-12L Inhibits Type I Interferon Production by Blocking the Interaction of Importin α and NF-κB Signaling Pathway.; Authors - Yisha Zhuo
human	WB	Viruses; Ivermectin Inhibits HBV Entry into the Nucleus by Suppressing KPNA2; Authors - Anna Nakanishi