KRAP/SSFA2 Polyklonaler Antikörper

KRAP/SSFA2 Polyklonal Antikörper für IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF

Konjugation

CoraLite®594 Fluorescent Dye

Kat-Nr. : CL594-14157

Synonyme

Cleavage signal 1 protein, CS 1, CS1, DKFZp313O1039, DKFZp779G0129, FLJ45996, KIAA1927, KRAP, KRAP/SSFA2, SPAG13, sperm specific antigen 2, SSFA2

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Galerie der Validierungsdaten

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  IF Staining of mouse pancreas using CL594-14157

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using CoraLite®594 KRAP/SSFA2 antibody (CL594-14157) at dilution of 1:200.

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  IF Staining of mouse pancreas using CL594-14157

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using CoraLite®594 KRAP/SSFA2 antibody (CL594-14157) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: Maus-Pankreasgewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-14157 bindet in IF KRAP/SSFA2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KRAP/SSFA2 fusion protein Ag5339
• Vollständiger Name: sperm specific antigen 2
• Berechnetes Molekulargewicht: 138 kDa
• GenBank-Zugangsnummer: BC028706
• Gene symbol: SSFA2
• Gene ID (NCBI): 6744
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KRAP (Ki-ras-induced actin-interacting protein), also known as SSFA2, CS1 or SPAG13, is a 1,259 amino acid cytoplasmic protein associated with filamentous-actin (F-actin). KRAP is strongly expressed in pancreas and testis. It is also present in colon cancer cell lines, and the expression is up-regulated by activated K-Ras. KRAP-deficient (KO) mice display decreased adiposity and improved glucose metabolism, implicating KRAP as a potential target for the prevention or treatment of obesity and related diseases. KRAP has also been suggested to be a potential target for cancer. This antibody recognizes endogenous KRAP, which migrates with an apparent molecular mass of 180 kDa in SDS-PAGE. (PMID: 14673706; 21501587; 21873152). This antibody is CL594(Ex/Em 593 nm/614 nm) conjugated.