KRAS-2B-specific Polyklonaler Antikörper

KRAS-2B-specific Polyklonal Antikörper für IF, IHC,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18294-1-AP

Synonyme

C K RAS, c Ki ras, GTPase KRas, K Ras 2, K RAS2B, K RAS4A, K RAS4B, KI RAS, KRAS, KRAS1, KRAS2, KRAS2A, KRAS2B, KRAS2B speicifc, Krasa, Krasb, NS3, RASK2

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Galerie der Validierungsdaten

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  IHC staining of human pancreas using 18294-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 18294-1-AP (KRAS-2B-specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 18294-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 18294-1-AP (KRAS-2B-specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas using 18294-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 18294-1-AP (KRAS-2B-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 18294-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 18294-1-AP (KRAS-2B-specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human stomach cancer using 18294-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 18294-1-AP (KRAS-2B-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach cancer using 18294-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 18294-1-AP (KRAS-2B-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach cancer using 18294-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 18294-1-AP (KRAS-2B-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach cancer using 18294-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 18294-1-AP (KRAS-2B-specific antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 18294-1-AP

  Immunofluorescent analysis of HepG2 cells, using KRAS antibody 18294-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

18294-1-AP bindet in IHC, IF,ELISA KRAS-2B-specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
• Berechnetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC013572
• Gene symbol: KRAS
• Gene ID (NCBI): 3845
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KRAS, also called p21, is a member of the Ras superfamily of proteins. It is located on human chromosome 12, and contains four coding exons and a 5' non-coding exon (PMID: 12778136). KRAS is a membrane-anchored guanosine triphosphate/guanosine diphosphate (GTP/GDP)-binding protein and is widely expressed in most human cells. Like other members of the Ras family, the KRAS protein is a GTPase, and it is involved in intracellular signal transduction and mainly responsible for EGFR-signaling activation (PMID: 19117687). KRAS mutations have been found in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. KRAS has two isoforms, 2A and 2B. The antibody is specific to KRAS, can’t recognize NRAS and HRAS.