Cytokeratin 1-specific Polyklonaler Antikörper

Cytokeratin 1-specific Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 16848-1-AP

Synonyme

67 kDa cytokeratin, CK 1, CK1, Cytokeratin 1, Cytokeratin 1-specific, EHK1, Hair alpha protein, keratin 1, KRT1, KRT1A, KRTA

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Galerie der Validierungsdaten

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  WB analysis using 16848-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16848-1-AP (Cytokeratin 1-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 16848-1-AP

  IP Result of anti-Cytokeratin 1-specific (IP:16848-1-AP, 3ug; Detection:16848-1-AP 1:500) with A431 cells lysate 3500ug.

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  IHC staining of human skin using 16848-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16848-1-AP (Cytokeratin 1-specific antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 16848-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16848-1-AP (Cytokeratin 1-specific antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human cervical cancer using 16848-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16848-1-AP (Cytokeratin 1-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 16848-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16848-1-AP (Cytokeratin 1-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 16848-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 16848-1-AP (Cytokeratin 1-specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 16848-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 16848-1-AP (Cytokeratin 1-specific antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A431 using 16848-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 1-specific antibody (16848-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 16848-1-AP

  Immunofluorescent analysis of A431 cells using 16848-1-AP (Cytokeratin 1-specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhautgewebe, mouse skin
• Erfolgreiche IP: A431-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautgewebe, humanes Lungenkarzinomgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 6 publications below

Produktinformation

16848-1-AP bindet in WB, IP, IHC, IF, ELISA Cytokeratin 1-specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: keratin 1
• Berechnetes Molekulargewicht: 66 kDa
• Beobachtetes Molekulargewicht: 67 kDa
• GenBank-Zugangsnummer: NM_006121
• Gene symbol: KRT1
• Gene ID (NCBI): 3848
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin-1 is type II keratin. It is a specific marker for terminal differentiation in mammalian epidermis. It is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. This antibody is specifically against ketatin I.

Publikationen

Species	Application	Title
rat	WB	Nat Commun; Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model; Authors - Mengyan Hu
human	WB,IF	J Invest Dermatol; Nuclear IL-33 plays an important role in the suppression of filaggrin, loricrin, keratin 1, and keratin 10 by IL-4 and IL-13 in human keratinocytes.; Authors - Xiuju Dai
human	WB	J Invest Dermatol; IL-22 downregulates peptidylarginine deiminase-1 in human keratinocytes: adding another piece to the IL-22 puzzle in epidermal barrier formation.; Authors - Avinash Padhi
mouse	WB,IF	Int J Mol Sci; Cabs1 Maintains Structural Integrity of Mouse Sperm Flagella during Epididymal Transit of Sperm.; Authors - Xiaoning Zhang
human	IF	Sci Rep; Loss-of-function Mutation in PMVK Causes Autosomal Dominant Disseminated Superficial Porokeratosis.; Authors - Jiuxiang Wang
human	WB	Front Endocrinol (Lausanne); c-Myc Upregulated by High Glucose Inhibits HaCaT Differentiation by S100A6 Transcriptional Activation.; Authors - Jie Zhang

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18343-1-AP