Cytokeratin 10 Polyklonaler Antikörper

Cytokeratin 10 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(19)

Rezensionen (1)

Kat-Nr. : 18343-1-AP

Synonyme

KRT10, CK 10, CK10, CK-10, Cytokeratin-10

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  WB analysis using 18343-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 18343-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin cancer using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin cancer using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin cancer using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of mouse skin using 18343-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 10 antibody (18343-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 18343-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 10 antibody (18343-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of A431 using 18343-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 10 (18343-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushautgewebe, A431-Zellen, Rattenhautgewebe
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Lungenkarzinomgewebe, humanes Hautkrebsgewebe, Rattenhautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushautgewebe
• Erfolgreiche Detektion in IF/ICC: A431-Zellen
• Erfolgreiche Detektion in FC (Intra): A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:20000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 5 publications below
• IF: See 11 publications below

Produktinformation

18343-1-AP bindet in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA Cytokeratin 10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 10 fusion protein Ag13136
• Vollständiger Name: keratin 10
• Berechnetes Molekulargewicht: 584 aa, 59 kDa
• Beobachtetes Molekulargewicht: 50-59 kDa
• GenBank-Zugangsnummer: BC034697
• Gene symbol: Cytokeratin 10
• Gene ID (NCBI): 3858
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. As a type I keratin, keratin 10 is a suprabasal marker of differentiation in stratified squamous epithelia.

Publikationen

Species	Application	Title
rat	IF	Cell Mol Biol Lett; The role of NPY2R/NFATc1/DYRK1A regulatory axis in sebaceous glands for sebum synthesis; Authors - Tao Yang
mouse	IF	Aging Cell; Nociceptive transient receptor potential canonical 7 (TRPC7) mediates aging-associated tumorigenesis induced by ultraviolet B.; Authors - Wen-Li Hsu
mouse	IHC	Life Sci; The potential of Diosgenin in treating psoriasis: Studies from HaCaT keratinocytes and imiquimod-induced murine model.; Authors - Shidi Wu
human	IF	mBio; Commensal-Related Changes in the Epidermal Barrier Function Lead to Alterations in the Benzo[a]Pyrene Metabolite Profile and Its Distribution in 3D Skin.; Authors - Lisa Lemoine
human	IF	Eur Surg Res; A novel combination of keratinocyte and autologous microskin grafting to repair full thickness skin loss; Authors - Kai Yin
mouse	IF	Int J Biol Macromol; Skin-adaptive film dressing with smart-release of growth factors accelerated diabetic wound healing; Authors - Yingzheng Zhao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Joshua (Verified Customer) (07-27-2019)

Cells differentiated at air-liquid interface. Cells fixed in 4% paraformaldehyde and stained at 4C overnight. Bright staining with minimal background.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human Keratinocytes

Cytokeratin 10 Antibody Immunofluorescence, validation (1:500 dilution) in Human Keratinocytes (Cat no:18343-1-AP)