Cytokeratin 10 Polyklonaler Antikörper
Cytokeratin 10 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 18343-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | Maushautgewebe, A431-Zellen, Rattenhautgewebe | 
| Erfolgreiche Detektion in IHC | humanes Zervixkarzinomgewebe,  humanes Mammakarzinomgewebe,  humanes Lungenkarzinomgewebe,  humanes Hautkrebsgewebe,  Rattenhautgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. | 
| Erfolgreiche Detektion in IF-P | Maushautgewebe | 
| Erfolgreiche Detektion in IF/ICC | A431-Zellen | 
| Erfolgreiche Detektion in FC (Intra) | A431-Zellen | 
Empfohlene Verdünnung
| Anwendung | Verdünnung | 
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 | 
| Immunhistochemie (IHC) | IHC : 1:500-1:2000 | 
| Immunfluoreszenz (IF)-P | IF-P : 1:50-1:500 | 
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 | 
| Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 3 publications below | 
| IHC | See 5 publications below | 
| IF | See 11 publications below | 
Produktinformation
18343-1-AP bindet in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA Cytokeratin 10 und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte | 
| In Publikationen genannte Reaktivität | human, Maus, Ratte | 
| Wirt / Isotyp | Kaninchen / IgG | 
| Klonalität | Polyklonal | 
| Typ | Antikörper | 
| Immunogen | Cytokeratin 10 fusion protein Ag13136 | 
| Vollständiger Name | keratin 10 | 
| Berechnetes Molekulargewicht | 584 aa, 59 kDa | 
| Beobachtetes Molekulargewicht | 50-59 kDa | 
| GenBank-Zugangsnummer | BC034697 | 
| Gene symbol | Cytokeratin 10 | 
| Gene ID (NCBI) | 3858 | 
| Konjugation | Unkonjugiert | 
| Form | Liquid | 
| Reinigungsmethode | Antigen-Affinitätsreinigung | 
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol | 
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. | 
Hintergrundinformationen
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. As a type I keratin, keratin 10 is a suprabasal marker of differentiation in stratified squamous epithelia.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for Cytokeratin 10 antibody 18343-1-AP | Protokoll herunterladen | 
| IHC protocol for Cytokeratin 10 antibody 18343-1-AP | Protokoll herunterladenl | 
| IF protocol for Cytokeratin 10 antibody 18343-1-AP | Protokoll herunterladen | 
| FC protocol for Cytokeratin 10 antibody 18343-1-AP | Download protocol | 
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen | 
Publikationen
| Species | Application | Title | 
|---|---|---|
| Cell Mol Biol Lett The role of NPY2R/NFATc1/DYRK1A regulatory axis in sebaceous glands for sebum synthesis | ||
| Aging Cell Nociceptive transient receptor potential canonical 7 (TRPC7) mediates aging-associated tumorigenesis induced by ultraviolet B. | ||
| Life Sci The potential of Diosgenin in treating psoriasis: Studies from HaCaT keratinocytes and imiquimod-induced murine model. | ||
| mBio Commensal-Related Changes in the Epidermal Barrier Function Lead to Alterations in the Benzo[a]Pyrene Metabolite Profile and Its Distribution in 3D Skin. | ||
| Eur Surg Res A novel combination of keratinocyte and autologous microskin grafting to repair full thickness skin loss | ||
| Int J Biol Macromol Skin-adaptive film dressing with smart-release of growth factors accelerated diabetic wound healing | 
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
| FH Joshua (Verified Customer) (07-27-2019) | Cells differentiated at air-liquid interface. Cells fixed in 4% paraformaldehyde and stained at 4C overnight. Bright staining with minimal background. 
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