Cytokeratin 15 Polyklonaler Antikörper

Cytokeratin 15 Polyklonal Antikörper für FC, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Kat-Nr. : 10137-1-AP

Synonyme

CK 15, CK15, Cytokeratin 15, K15, K1CO, keratin 15, KRT15, KRTB

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Galerie der Validierungsdaten

• 

  WB analysis of A431 using 10137-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10137-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10137-1-AP (Cytokeratin 15 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10137-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10137-1-AP (Cytokeratin 15 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 10137-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 10137-1-AP

  IP Result of anti-Cytokeratin 15 (IP:10137-1-AP, 4ug; Detection:10137-1-AP 1:500) with A431 cells lysate 3040ug.

• 

  IHC staining of human cervical cancer using 10137-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 10137-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 10137-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10137-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10137-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 10137-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of A431 using 10137-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.2 ug Anti-Human Cytokeratin 15 (10137-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, Maus-Thymusgewebe, PC-3-Zellen
• Erfolgreiche IP: A431-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Ösophaguskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 6 publications below
• IF: See 9 publications below

Produktinformation

10137-1-AP bindet in WB, IP, IHC, IF, FC, ELISA Cytokeratin 15 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 15 fusion protein Ag0185
• Vollständiger Name: keratin 15
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC002641
• Gene symbol: KRT15
• Gene ID (NCBI): 3866
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 15 is a type I cytokeratin. It is found in some progenitor basal cells within complex epithelia.

Publikationen

Species	Application	Title
mouse	IHC	Biomater Sci; A hybrid hydrogel composed of chitin and β-glucan for the effective management of wound healing and scarring; Authors - Ximeng Kang
mouse	IF	Stem Cells; LIM Homeobox Domain 2 (Lhx2) is required for corneal epithelial homeostasis.; Authors - R Sartaj
human	IF	J Invest Dermatol; GW bodies: cytoplasmic compartments in normal human skin.; Authors - Zee Jennifer M JM
human	WB	Carcinogenesis; Survival Of Skin Cancer Stem Cells Requires The Ezh2 Polycomb Group Protein.; Authors - Gautam Adhikary
mouse	IF	Sci Rep; Characterization of slow cycling corneal limbal epithelial cells identifies putative stem cell markers.; Authors - R Sartaj
rat	WB,IF	Front Med (Lausanne); Metformin Promotes Mechanical Stretch-Induced Skin Regeneration by Improving the Proliferative Activity of Skin-Derived Stem Cells.; Authors - Shaoheng Xiong