Cytokeratin 15 Monoklonaler Antikörper

Cytokeratin 15 Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

6B4F8

Kat-Nr. : 60247-1-Ig

Synonyme

CK 15, CK15, Cytokeratin 15, K15, K1CO, keratin 15, KRT15, KRTB

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Galerie der Validierungsdaten

• 

  WB analysis of A431 using 60247-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 60247-1-Ig (Cytokeratin 15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human oesophagus cancer using 60247-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60247-1-Ig (Cytokeratin 15 antibody at dilution of 1:500 (under 10x lens).

• 

  IHC staining of human oesophagus cancer using 60247-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60247-1-Ig (Cytokeratin 15 antibody at dilution of 1:500 (under 40x lens).

• 

  IHC staining of human skin cancer using 60247-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60247-1-Ig (Cytokeratin 15 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin cancer using 60247-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60247-1-Ig (Cytokeratin 15 antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 60247-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 15 antibody (60247-1-Ig, Clone: 6B4F8 ) at dilution of 1:25 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of A431 using 60247-1-Ig

  1X10^6 A431 cells were intracellularly stained with 0.2 ug Anti-Human Cytokeratin 15 (60247-1-Ig, Clone:6B4F8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen
• Erfolgreiche Detektion in IHC: humanes Ösophaguskarzinomgewebe, humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:100-1:1000
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

60247-1-Ig bindet in WB, IHC, IF, FC, ELISA Cytokeratin 15 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 15 fusion protein Ag0185
• Vollständiger Name: keratin 15
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC002641
• Gene symbol: KRT15
• Gene ID (NCBI): 3866
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 15 is a type I cytokeratin. It is found in some progenitor basal cells within complex epithelia.

Publikationen

Species	Application	Title
	IF	Oxid Med Cell Longev; Exosomes Derived from Dermal Papilla Cells Mediate Hair Follicle Stem Cell Proliferation through the Wnt3a/β-Catenin Signaling Pathway; Authors - Jiali Li