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Cytokeratin 17-Specific Polyklonaler Antikörper
Cytokeratin 17-Specific Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IHC, IF/ICC, IP, CoIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 17516-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | A431-Zellen, HaCaT-Zellen, HeLa-Zellen, Maushautgewebe, Rattenhautgewebe |
Erfolgreiche IP | A431-Zellen |
Erfolgreiche Detektion in IHC | human cervical squamous cancer tissue, human bowen disease tissue, human lung squamous cell carcinoma tissue Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF/ICC | HepG2-Zellen, HeLa-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:1000-1:20000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:4000-1:16000 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:300-1:1200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 3 publications below |
WB | See 16 publications below |
IHC | See 12 publications below |
IF | See 7 publications below |
CoIP | See 1 publications below |
Produktinformation
17516-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA Cytokeratin 17-Specific und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Maus |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | Peptid |
Vollständiger Name | keratin 17 |
Berechnetes Molekulargewicht | 48 kDa |
Beobachtetes Molekulargewicht | 48 kDa |
GenBank-Zugangsnummer | NM_000422 |
Gene symbol | Cytokeratin 17 |
Gene ID (NCBI) | 3872 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 17 is a type I cytokeratin. It is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. It is used as a marker for trauma. This antibody is specifically against KRT17.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for Cytokeratin 17-Specific antibody 17516-1-AP | Protokoll herunterladen |
IHC protocol for Cytokeratin 17-Specific antibody 17516-1-AP | Protokoll herunterladenl |
IF protocol for Cytokeratin 17-Specific antibody 17516-1-AP | Protokoll herunterladen |
IP protocol for Cytokeratin 17-Specific antibody 17516-1-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Clin Cancer Res A Preclinical Trial and Molecularly Annotated Patient Cohort Identify Predictive Biomarkers in Homologous Recombination-deficient Pancreatic Cancer. | ||
Breast Cancer Res Anillin regulates breast cancer cell migration, growth, and metastasis by non-canonical mechanisms involving control of cell stemness and differentiation. | ||
Oncogene The keratin 17/YAP/IL6 axis contributes to E-cadherin loss and aggressiveness of diffuse gastric cancer
| ||
Phytother Res Epigallocatechin-3-gallate promotes wound healing response in diabetic mice by activating keratinocytes and promoting re-epithelialization | ||
J Invest Dermatol Keratin 17 Impacts Global Gene Expression and Controls G2/M Cell Cycle Transition in Ionizing Radiation-Induced Skin Damage |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Lindsey (Verified Customer) (08-08-2025) | This antibody works very well on both FFPE tissue (with heat based citrate buffer antigen retrieval) and whole mount embryonic skin. It is highly specific based on our experience and can be used from 1/1000 to 1/1200 dilution (1/1000 for adult and 1/1200 for embryonic).
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