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Cytokeratin 17-Specific Polyklonaler Antikörper

Cytokeratin 17-Specific Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(29)

Kat-Nr. : 17516-1-AP

Synonyme

Cytokeratin 17, KRT17, CK 17, CK-17, Cytokeratin,Keratin



Geprüfte Anwendungen

Erfolgreiche Detektion in WBA431-Zellen, HaCaT-Zellen, HeLa-Zellen, Maushautgewebe, Rattenhautgewebe
Erfolgreiche IPA431-Zellen
Erfolgreiche Detektion in IHChuman cervical squamous cancer tissue, human bowen disease tissue, human lung squamous cell carcinoma tissue
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:20000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:4000-1:16000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

17516-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA Cytokeratin 17-Specific und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name keratin 17
Berechnetes Molekulargewicht 48 kDa
Beobachtetes Molekulargewicht 48 kDa
GenBank-ZugangsnummerNM_000422
Gene symbol Cytokeratin 17
Gene ID (NCBI) 3872
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 17 is a type I cytokeratin. It is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. It is used as a marker for trauma. This antibody is specifically against KRT17.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for Cytokeratin 17-Specific antibody 17516-1-APProtokoll herunterladen
IHC protocol for Cytokeratin 17-Specific antibody 17516-1-APProtokoll herunterladenl
IF protocol for Cytokeratin 17-Specific antibody 17516-1-APProtokoll herunterladen
IP protocol for Cytokeratin 17-Specific antibody 17516-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouse

Science

Spatiotemporal immune zonation of the human kidney.

Authors - Benjamin J Stewart
humanIHC

Clin Cancer Res

A Preclinical Trial and Molecularly Annotated Patient Cohort Identify Predictive Biomarkers in Homologous Recombination-deficient Pancreatic Cancer.

Authors - Yifan Wang
humanWB

Breast Cancer Res

Anillin regulates breast cancer cell migration, growth, and metastasis by non-canonical mechanisms involving control of cell stemness and differentiation.

Authors - Dongdong Wang
humanWB

Oncogene

The keratin 17/YAP/IL6 axis contributes to E-cadherin loss and aggressiveness of diffuse gastric cancer

Authors - Mengjie Li
  • KD Validated
mouseWB

Phytother Res

Epigallocatechin-3-gallate promotes wound healing response in diabetic mice by activating keratinocytes and promoting re-epithelialization

Authors - Yongling Ning
mouse,humanWB

J Invest Dermatol

Keratin 17 Impacts Global Gene Expression and Controls G2/M Cell Cycle Transition in Ionizing Radiation-Induced Skin Damage

Authors - ZhiCao Yue

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Lindsey (Verified Customer) (08-08-2025)

This antibody works very well on both FFPE tissue (with heat based citrate buffer antigen retrieval) and whole mount embryonic skin. It is highly specific based on our experience and can be used from 1/1000 to 1/1200 dilution (1/1000 for adult and 1/1200 for embryonic).

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Mouse skin