Cytokeratin 18 Monoklonaler Antikörper

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• KD/KO Validated

Cytokeratin 18 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(23)

CloneNo.

1G11C4

Kat-Nr. : 66187-1-Ig

Synonyme

KRT18, 1G11C4, Cell proliferation-inducing gene 46 protein, CK 18, CK18

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  WB analysis using 66187-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66187-1-Ig

  WB result of Cytokeratin 18 antibody (66187-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytokeratin 18 transfected HeLa cells.

• 

  WB analysis of pig skin using 66187-1-Ig

  pig skin tissue were subjected to SDS PAGE followed by western blot with 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 66187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66187-1-Ig (Cytokeratin 18 antibody at dilution of 1:1200 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human kidney using 66187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66187-1-Ig (Cytokeratin 18 antibody at dilution of 1:1200 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human liver using 66187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:2400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 66187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:1 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 66187-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 66187-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:3200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 66187-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 66187-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 66187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:2400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 66187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:2400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:2400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:2400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 66187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:2400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 66187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:2400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using 66187-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Cytokeratin 18 antibody (66187-1-Ig, Clone: 1G11C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse testis using 66187-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Cytokeratin 18 antibody (66187-1-Ig, Clone: 1G11C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of A549 using 66187-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Cytokeratin 18 antibody (66187-1-Ig, Clone: 1G11C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 66187-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 18 antibody (66187-1-Ig, Clone: 1G11C4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HUVEC using 66187-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HUVEC cells using Cytokeratin 18 antibody (66187-1-Ig, Clone: 1G11C4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HeLa using 66187-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 18 antibody (66187-1-Ig, Clone: 1G11C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

• 

  FC experiment of HeLa using 66187-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 18 (66187-1-Ig, Clone:1G11C4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, 4T1-Zellen, A431-Zellen, COLO 320-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen, Hausschwein-Hautgewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Lebergewebe, humanes Lungenkarzinomgewebe, humanes Nierenzellkarzinomgewebe, humanes Tonsillitisgewebe, Mauslebergewebe, Rattenlebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushodengewebe
• Erfolgreiche Detektion in IF/ICC: A549-Zellen, HeLa-Zellen, HUVEC-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:20000-1:100000
• Immunhistochemie (IHC): IHC : 1:600-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 6 publications below
• IF: See 13 publications below

Produktinformation

66187-1-Ig bindet in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA Cytokeratin 18 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 18 fusion protein Ag1260
• Vollständiger Name: keratin 18
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC000180
• Gene symbol: Cytokeratin 18
• Gene ID (NCBI): 3875
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.

Publikationen

Species	Application	Title
mouse	IF	Nat Cell Biol; Blebbisomes are large, organelle-rich extracellular vesicles with cell-like properties; Authors - Dennis K Jeppesen
mouse	IF	Redox Biol; ACSL4 deficiency confers protection against ferroptosis-mediated acute kidney injury.; Authors - Yue Wang
human	IF	J Pathol; Histone demethylase PHF8 drives neuroendocrine prostate cancer progression by epigenetically upregulating FOXA2.; Authors - Qiuli Liu
human	WB	Hum Reprod; T-cadherin inhibits invasion and migration of endometrial stromal cells in endometriosis.; Authors - Qinsheng Lu
mouse	IF	Stem Cell Res Ther; Expansion of mouse castration-resistant intermediate prostate stem cells in vitro; Authors - Yalan Xu
human	IHC,IF	Commun Biol; Single-cell analysis supports a luminal-neuroendocrine transdifferentiation in human prostate cancer.; Authors - Baijun Dong