Cytokeratin 19 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Cytokeratin 19 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 14965-1-AP

Synonyme

Cell and organelle markers, CK 19, CK19, Cytokeratin 19, Cytoskeleton Marker, K19, K1CS, keratin 19, KRT19

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Galerie der Validierungsdaten

• 

  WB analysis of MCF-7 using 14965-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14965-1-AP

  WB result of KRT19 antibody (14965-1-AP, 1:50,000) with si-Control and si-KRT19 transfected HeLa cells.

• 

  WB analysis of HepG2 using 14965-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 14965-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:6400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:6400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of rat colon using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14965-1-AP (Cytokeratin 19 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human cervical cancer using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14965-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cytokeratin 19 antibody (14965-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 14965-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 19 antibody (14965-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

• 

  FC experiment of MCF-7 using 14965-1-AP

  1X10^6 MCF-7 cells were stained with 0.2 ug Anti-Human Cytokeratin 19 (14965-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF7 cells, BxPC-3-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Leberkarzinomgewebe, humanes Lungenkarzinomgewebe, humanes Zervixkarzinomgewebe, Maus-Kolongewebe, Ratten-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, A431-Zellen
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:1000-1:6400
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 5 publications below
• IF: See 4 publications below

Produktinformation

14965-1-AP bindet in WB, IHC, IF, FC, ELISA Cytokeratin 19 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 19 fusion protein Ag6830
• Vollständiger Name: keratin 19
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 40 kDa, 46 kDa
• GenBank-Zugangsnummer: BC002539
• Gene symbol: KRT19
• Gene ID (NCBI): 3880
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT19, one of type I keratins, is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Due to its high sensitivity, KRT19 is the most used marker for detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients. This antibody, generated against full length KRT19 protein, also recognizes KRT17, another type I keratin homologous to KRT19.

Publikationen

Species	Application	Title
mouse	IF	Gastroenterology; ECM1 Prevents Activation of Transforming Growth Factor β, Hepatic Stellate Cells, and Fibrogenesis in Mice.; Authors - Weiguo Fan
mouse	IF	Hepatology; Cholestasis-induced adaptive remodeling of interlobular bile ducts.; Authors - Nachiket Vartak
human	IHC	Cell Rep; In Vivo Functional Platform Targeting Patient-Derived Xenografts Identifies WDR5-Myc Association as a Critical Determinant of Pancreatic Cancer.; Authors - Alessandro Carugo
mouse	IHC	Arch Toxicol; Galunisertib modifies the liver fibrotic composition in the Abcb4Ko mouse model.; Authors - Seddik Hammad
mouse	IF	Front Cell Dev Biol; Volumetric imaging reveals VEGF-C-dependent formation of hepatic lymph vessels in mice; Authors - Stefanie Bobe
monkey	IF	Biochim Biophys Acta Mol Basis Dis; The zonula occludens protein family regulates the hepatic barrier system in the murine liver.; Authors - Masahiko Itoh

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