Cytokeratin 2e Polyklonaler Antikörper

Cytokeratin 2e Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21725-1-AP

Synonyme

CK 2e, Cytokeratin 2e, Epithelial keratin 2e, K2e, keratin 2, Keratin 2 epidermis, Keratin 2e, KRT2, KRT2A, KRT2E, KRTE, Type II keratin Kb2

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Galerie der Validierungsdaten

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  WB analysis using 21725-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21725-1-AP (KRT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 21725-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 21725-1-AP (KRT2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 21725-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21725-1-AP (KRT2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 21725-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21725-1-AP (KRT2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 21725-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 21725-1-AP (KRT2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 21725-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 21725-1-AP (KRT2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, Maushautgewebe
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Ösophaguskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

21725-1-AP bindet in WB, IHC, ELISA Cytokeratin 2e und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 2e fusion protein Ag16384
• Vollständiger Name: keratin 2
• Berechnetes Molekulargewicht: 639 aa, 66 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC096294
• Gene symbol: KRT2
• Gene ID (NCBI): 3849
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18).Keratin 2 is a type II cytokeratin. It is found largely in the upper spinous layer of epidermal keratinocytes, and also in other regions, notably the epidermis covering the knee, thigh, and groin.