Cytokeratin 6A-specific Polyklonaler Antikörper

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• KD/KO Validated

Cytokeratin 6A-specific Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16853-1-AP

Synonyme

Cytokeratin 6, KRT6A, CK-6D, CK-6A, CK6A

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  WB analysis of A431 using 16853-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 16853-1-AP (Cytokeratin 6A-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of AML12 using 16853-1-AP

  WB result of Cytokeratin 6A antibody (16853-1-AP; 1:600; room temperature for 1.5 hours) with wild-type and Cytokeratin 6A knockout AML 12 cells.

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  WB analysis of human saliva using 16853-1-AP

  human saliva were subjected to SDS PAGE followed by western blot with 16853-1-AP (Cytokeratin 6A-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16853-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16853-1-AP (Cytokeratin 6A-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 16853-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 16853-1-AP (Cytokeratin 6A-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16853-1-AP

  IP result of anti-Cytokeratin 6A-specific (IP:16853-1-AP, 4ug; Detection:16853-1-AP 1:300) with HeLa cells lysate 520ug.

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  IHC staining of human skin cancer using 16853-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 16853-1-AP (Cytokeratin 6A-specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 16853-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 16853-1-AP (Cytokeratin 6A-specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bowen disease using 16853-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 16853-1-AP (Cytokeratin 6A-specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bowen disease using 16853-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 16853-1-AP (Cytokeratin 6A-specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, Maushautgewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe, human bowen disease; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16853-1-AP bindet in WB, IHC, IP, ELISA Cytokeratin 6A-specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: keratin 6A
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: NM_005554
• Gene symbol: Cytokeratin 6A
• Gene ID (NCBI): 3853
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 6 is a type II keratin. It is used as marker for epidermal hyperproliferation and differentiation. Three keratin 6 isoforms were found, ketatin 6A,6B and 6C. They share more than 99% identical DNA sequence. This antibody is against all three isoforms of keratin 6, but it does not bind other keratins.