KTN1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

KTN1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Rezensionen (1)

Kat-Nr. : 19841-1-AP

Synonyme

CG 1 antigen, CG1, KIAA0004, Kinectin, kinectin 1 (kinesin receptor), Kinesin receptor, KNT, KTN1, MU RMS 40.19

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Galerie der Validierungsdaten

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  WB analysis using 19841-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19841-1-AP (KTN1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 19841-1-AP

  WB result of KTN1 antibody (19841-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KTN1 transfected A549 cells.

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  IP experiment of Jurkat using 19841-1-AP

  IP Result of anti-KTN1 (IP:19841-1-AP, 4ug; Detection:19841-1-AP 1:500) with Jurkat cells lysate 1200ug.

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  IHC staining of human ovary tumor using 19841-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 19841-1-AP (KTN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 19841-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19841-1-AP (KTN1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 19841-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19841-1-AP (KTN1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 19841-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KTN1 antibody (19841-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 19841-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KTN1 antibody (19841-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, Jurkat-Zellen, K-562-Zellen, PC-3-Zellen, SKOV-3-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

19841-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA KTN1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Affe, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KTN1 fusion protein Ag13854
• Vollständiger Name: kinectin 1 (kinesin receptor)
• Berechnetes Molekulargewicht: 1357 aa, 156 kDa
• Beobachtetes Molekulargewicht: 156 kDa
• GenBank-Zugangsnummer: BC117132
• Gene symbol: KTN1
• Gene ID (NCBI): 3895
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KTN1/Kinectin is an integral endoplasmic reticulum (ER) membrane protein which acts as dynamic anchor to facilitate ER membrane extension along microtubules. It has been found to bind kinesin, Rho GTPase, and translation elongation factor-1delta and gets involved in organelle motility and protein synthesis.

Publikationen

Species	Application	Title
human,monkey	WB	Nature; ER proteins decipher the tubulin code to regulate organelle distribution; Authors - Pengli Zheng ; KO Validated
human,mouse	WB	Sci Transl Med; CPEB alteration and aberrant transcriptome-polyadenylation lead to a treatable SLC19A3 deficiency in Huntington's disease.; Authors - Sara Picó
human	IF	Cell Res; DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.; Authors - Pengli Zheng
	IF	EMBO Rep; Mobile late endosomes modulate peripheral endoplasmic reticulum network architecture.; Authors - Menno Spits
human	WB, IHC	Clin Sci (Lond); The long noncoding RNA KTN1-AS1 promotes bladder cancer tumorigenesis via KTN1 cis-activation and the consequent initiation of Rho GTPase-mediated signaling.; Authors - Xueying Hu
monkey	WB	Curr Biol; ATL3 Is a Tubular ER-Phagy Receptor for GABARAP-Mediated Selective Autophagy.; Authors - Qingzhou Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Siting (Verified Customer) (01-28-2020)

This antibody worked well with western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart tissue