CACNA1C Polyklonaler Antikörper

CACNA1C Polyklonal Antikörper für WB, IHC, IF-P, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF-P, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(27)

Rezensionen (1)

Kat-Nr. : 21774-1-AP

Synonyme

CACH2, CACN2, CACNL1A1, Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle, CCHL1A1

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  WB analysis using 21774-1-AP

  Mouse heart lysate was subjected to SDS PAGE, followed by western blot with 21774-1-AP (CACNA1C antibody) at a dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 21774-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21774-1-AP (L-VOCC antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 21774-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21774-1-AP (L-VOCC antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 21774-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 21774-1-AP (L-VOCC antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse liver using 21774-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 21774-1-AP (L-VOCC antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of rat brain using 21774-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21774-1-AP (L-VOCC Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of rat brain using 21774-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21774-1-AP (L-VOCC Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of mouse brain using 21774-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using L-VOCC antibody (21774-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 21774-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using L-VOCC antibody (21774-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 21774-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human L-VOCC (21774-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, Mauslebergewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 21 publications below
• IHC: See 2 publications below
• IF: See 6 publications below

Produktinformation

21774-1-AP bindet in WB, IHC, IF-P, FC (Intra), ELISA CACNA1C und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CACNA1C fusion protein Ag16455
• Vollständiger Name: calcium channel, voltage-dependent, L type, alpha 1C subunit
• Berechnetes Molekulargewicht: 249 kDa
• Beobachtetes Molekulargewicht: 249 kDa
• GenBank-Zugangsnummer: BC146846
• Gene symbol: CACNA1C
• Gene ID (NCBI): 775
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Calcium voltage-gated channel subunit alpha1 C (CACNA1C, also known as CACH2 and Cav1.2) couples transient activation of inward calcium current to transcriptional regulation and plays an important role in dendritic development, neuronal survival, synaptic plasticity, memory formation, learning, and behavior (PMID: 21248242;  16251435; 20169575; 19047462; 18174367). Genetic variation in CACNA1C has also been associated with depression, schizophrenia, and autism spectrum disorders, as well as changes in brain function and structure in control subjects who have no diagnosable psychiatric illness (PMID: 22705413).

Publikationen

Species	Application	Title
rat	WB	Phytomedicine; Protective role and mechanism of snakegourd peel against myocardial infarction in rats.; Authors - Guanlin Yang
mouse	WB	Antioxid Redox Signal; Anthracycline-induced atrial structural and electrical remodeling characterizes early cardiotoxicity and contributes to atrial conductive instability and dysfunction.; Authors - Ruopeng Tan
rat	WB	Front Immunol; Interleukin-6-Mediated-Ca2+ Handling Abnormalities Contributes to Atrial Fibrillation in Sterile Pericarditis Rats.; Authors - Jie Liao
human	WB	Reprod Biol Endocrinol; In vitro fertilization with frozen embryo transfer increased histamine-mediated contractile sensitivity via PKCβ in human umbilical vein; Authors - Jiaqi Tang
rat	WB	Mol Nutr Food Res; Quercetin Attenuates Ethanol-Induced Iron Uptake and Myocardial Injury by Regulating the Angiotensin II-L-Type Calcium Channel.; Authors - Xiaoping Guo
mouse,human	WB	Commun Biol; Cytosolic peptides encoding CaV1 C-termini downregulate the calcium channel activity-neuritogenesis coupling.; Authors - Yaxiong Yang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wu (Verified Customer) (07-16-2021)

Works good for IHC assay

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: glandular lumen

CACNA1C Antibody Immunohistochemistry validation (1:100 dilution) in glandular lumen (Cat no:21774-1-AP)