L1CAM/CD171 Monoklonaler Antikörper

L1CAM/CD171 Monoklonal Antikörper für IHC

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

IHC

Konjugation

Biotin

Publikationen(1)

CloneNo.

3H7B9

Kat-Nr. : Biotin-67115

Synonyme

L1CAM, 3H7B9, CAML1, CD171, HSAS

---

• 

  IHC staining of mouse brain using Biotin-67115

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Biotin-67115 (L1CAM antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hypothalamus using Biotin-67115

  Immunohistochemical analysis of paraffin-embedded human hypothalamus tissue slide using Biotin-67115 (L1CAM antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hypothalamus using Biotin-67115

  Immunohistochemical analysis of paraffin-embedded human hypothalamus tissue slide using Biotin-67115 (L1CAM antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Hypothalamus-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

Biotin-67115 bindet in IHC L1CAM/CD171 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: L1CAM/CD171 fusion protein Ag17706
• Vollständiger Name: L1 cell adhesion molecule
• Berechnetes Molekulargewicht: 1257 aa, 140 kDa
• Beobachtetes Molekulargewicht: 220 kDa
• GenBank-Zugangsnummer: BC126229
• Gene symbol: L1CAM
• Gene ID (NCBI): 3897
• Konjugation: Biotin
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503).

Publikationen

Species	Application	Title
		Int J Mol Sci; Neuronally Derived Extracellular Vesicles' Oligomeric and p129-α-Synuclein Levels for Differentiation of Parkinson's Disease from Essential Tremor; Authors - Costanza Maria Cristiani