LACTB2 Monoklonaler Antikörper

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• KD/KO Validated

LACTB2 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

1G6B1

Kat-Nr. : 67399-1-Ig

Synonyme

EC:3.1.27.-, CGI-83, CGI83, CGI 83, Beta-lactamase-like protein 2

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  WB analysis of A549 using 67399-1-Ig

  WB result of LACTB2 antibody (67399-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LACTB2 transfected A549 cells.

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  WB analysis using 67399-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67399-1-Ig (LACTB2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 67399-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67399-1-Ig (LACTB2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 67399-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67399-1-Ig (LACTB2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67399-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67399-1-Ig (LACTB2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human liver cancer using 67399-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using LACTB2 antibody (67399-1-Ig, Clone: 1G6B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human liver cancer using 67399-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using LACTB2 antibody (67399-1-Ig, Clone: 1G6B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse liver using 67399-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using LACTB2 antibody (67399-1-Ig, Clone: 1G6B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 67399-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using LACTB2 antibody (67399-1-Ig, Clone: 1G6B1 ) at dilution of 1:2000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Leberkarzinomgewebe, Mauslebergewebe
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:300-1:1200
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67399-1-Ig bindet in WB, IHC, IF/ICC, IF-P, ELISA LACTB2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LACTB2 fusion protein Ag10231
• Vollständiger Name: lactamase, beta 2
• Berechnetes Molekulargewicht: 288 aa, 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC000878
• Gene symbol: LACTB2
• Gene ID (NCBI): 51110
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.