LAMC1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

LAMC1 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Ratte und mehr (1)

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

CloneNo.

1G2G4

Kat-Nr. : 67706-1-Ig

Synonyme

Laminin subunit gamma 1, 1G2G4, LAMB2, LAMC 1, Laminin subunit gamma-1

---

• 

  WB analysis using 67706-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67706-1-Ig (LAMC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67706-1-Ig

  WB result of LAMC1 antibody (67706-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LAMC1 transfected HeLa cells.

• 

  WB analysis of human placenta using 67706-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67706-1-Ig (LAMC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 67706-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67706-1-Ig (LAMC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat rectum using 67706-1-Ig

  rat rectum tissue were subjected to SDS PAGE followed by western blot with 67706-1-Ig (LAMC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig rectum using 67706-1-Ig

  pig rectum tissue were subjected to SDS PAGE followed by western blot with 67706-1-Ig (LAMC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 67706-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LAMC1 antibody (67706-1-Ig, Clone: 1G2G4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, humanes Plazenta-Gewebe, JAR-Zellen, LNCaP-Zellen, Hausschwein-Rektumgewebe, Ratten-Rektumgewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

67706-1-Ig bindet in WB, IF/ICC, ELISA LAMC1 und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LAMC1 fusion protein Ag14674
• Vollständiger Name: laminin, gamma 1 (formerly LAMB2)
• Berechnetes Molekulargewicht: 178 kDa
• Beobachtetes Molekulargewicht: 200-220 kDa
• GenBank-Zugangsnummer: BC015586
• Gene symbol: LAMC1
• Gene ID (NCBI): 3915
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat	WB	Neuroscience; Comparative Proteomics of Rat Olfactory Bulb Reveal Insights into Susceptibility and Resiliency to Chronic-stress-induced Depression or Anxiety.; Authors - Dan Liu
human	WB	Rheumatology (Oxford); The central helicase domain holds the major conformational epitopes of melanoma differentiation-associated gene 5 autoantibodies; Authors - Yongxin Mo
rat,mouse	WB,IF	Exp Neurol; LAMC1 attenuates neuronal apoptosis via FAK/PI3K/AKT signaling pathway after subarachnoid hemorrhage; Authors - Qiaowei Wu
human	WB	J Transl Med; HIF-1α-mediated LAMC1 overexpression is an unfavorable predictor of prognosis for glioma patients: evidence from pan-cancer analysis and validation experiments; Authors - Jianrong Bai