LAMC3-Specific Monoklonaler Antikörper

LAMC3-Specific Monoklonal Antikörper für WB, IF-P, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus

Anwendung

WB, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

4B8C5

Kat-Nr. : 67261-1-Ig

Synonyme

LAMC3, 4B8C5, Laminin 12 subunit gamma, Laminin 14 subunit gamma, Laminin 15 subunit gamma

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  WB analysis of human testis using 67261-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 67261-1-Ig (LAMC3-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 67261-1-Ig

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 67261-1-Ig (LAMC3-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 67261-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 67261-1-Ig (LAMC3-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of mouse testis using 67261-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using LAMC3-Specific antibody (67261-1-Ig, Clone: 4B8C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  Affinity and Kinetic Characterization of 67261-1-Ig

  Biolayer interferometry (BLl) kinetic assays of 67261-1-PBS against Human GRP78/BIP were performed. The affinity constant is 0.559 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe, Maus-Plazenta-Gewebe, PC-3-Zellen
• Erfolgreiche Detektion in IF-P: Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

67261-1-Ig bindet in WB, IF-P, ELISA LAMC3-Specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LAMC3-Specific fusion protein Ag19568
• Vollständiger Name: laminin, gamma 3
• Berechnetes Molekulargewicht: 1575 aa, 171 kDa
• Beobachtetes Molekulargewicht: 180-200 kDa
• GenBank-Zugangsnummer: NM_006059
• Gene symbol: LAMC3
• Gene ID (NCBI): 10319
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	bioRxiv; Laminin β4 is required for the development of human peripheral sensory neurons; Authors - Kenyi Saito-Diaz