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CD107b / LAMP2 Monoklonaler Antikörper
CD107b / LAMP2 Monoklonal Antikörper für WB, IHC, ELISA
Wirt / Isotyp
Maus / IgA
Getestete Reaktivität
Hausschwein, human, Maus und mehr (2)
Anwendung
WB, IHC, IP, CoIP, ELISA
Konjugation
Unkonjugiert
CloneNo.
2D3B9
Kat-Nr. : 66301-1-Ig
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | L02-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, HUVEC-Zellen, Jurkat-Zellen, Mauslebergewebe, Hausschwein-Lebergewebe |
Erfolgreiche Detektion in IHC | humanes Leberkarzinomgewebe, humanes Nierengewebe, humanes Lebergewebe, humanes Lungenkarzinomgewebe, Mauslebergewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunhistochemie (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 5 publications below |
WB | See 87 publications below |
IHC | See 3 publications below |
IF | See 1 publications below |
IP | See 2 publications below |
CoIP | See 2 publications below |
Produktinformation
66301-1-Ig bindet in WB, IHC, IP, CoIP, ELISA CD107b / LAMP2 und zeigt Reaktivität mit Hausschwein, human, Maus
Getestete Reaktivität | Hausschwein, human, Maus |
In Publikationen genannte Reaktivität | human, Hausschwein, Huhn, Maus, Rind |
Wirt / Isotyp | Maus / IgA |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | CD107b / LAMP2 fusion protein Ag0595 |
Vollständiger Name | lysosomal-associated membrane protein 2 |
Berechnetes Molekulargewicht | 45 kDa |
Beobachtetes Molekulargewicht | 120 kDa |
GenBank-Zugangsnummer | BC002965 |
Gene symbol | LAMP2 |
Gene ID (NCBI) | 3920 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Thiophiler Affinitätschromatograph |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Lysosomal-associated membrane protein 2 (LAMP2, synonyms: LAMPB, CD107b) is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is a ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for CD107b / LAMP2 antibody 66301-1-Ig | Protokoll herunterladen |
IHC protocol for CD107b / LAMP2 antibody 66301-1-Ig | Protokoll herunterladenl |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Mol Cell Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis. | ||
Autophagy SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes | ||
Arthritis Rheumatol LAMP3 induces lysosome-dependent cell death by impairing autophagic caspase-8 degradation in Sjögren's salivary glands | ||
J Clin Invest TMEM25 modulates neuronal excitability and NMDA receptor subunit NR2B degradation. | ||
Redox Biol WBP2 restrains the lysosomal degradation of GPX4 to inhibit ferroptosis in cisplatin-induced acute kidney injury |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH X (Verified Customer) (10-17-2024) | It can detect endo LAMP2 at the right MW, but also with some other bands nearby. Need optimization.
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FH Laura (Verified Customer) (04-08-2024) | Antibody works nicely in ICC-IF and WB in different cell lines of intestine and pancreas origin.
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FH LENIN (Verified Customer) (06-12-2022) | Antibody works very well for culture cells and Western blots.
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FH NYDIA (Verified Customer) (03-12-2022) | This antibody works great for immunofluorescence. No background. I highly recommend this product.
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FH Eric (Verified Customer) (03-13-2021) | I had success with some and others not. I used microglia and I did not get strong expression but in macrophages I got better bands with same dilution and treatment. Many nonspecific bands
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FH Tanusree (Verified Customer) (12-03-2019) | This antibody works good in western blotting analysis using mouse tissues.
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FH miguel (Verified Customer) (12-11-2018) | The antibody was used with an inhouse protocol for Immunohistochemistry. The results varied from good to great for both stains and imaging.
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