LAMP3 Polyklonaler Antikörper

LAMP3 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

20

Rezensionen (1)

Kat-Nr. : 12632-1-AP

Synonyme

CD208, DC LAMP, DCLAMP, LAMP, LAMP 3, LAMP3, Protein TSC403, TSC403

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Galerie der Validierungsdaten

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  WB analysis using 12632-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12632-1-AP (LAMP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12632-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12632-1-AP (LAMP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 12632-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12632-1-AP (LAMP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 12632-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12632-1-AP (LAMP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 12632-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12632-1-AP (LAMP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat lung using 12632-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 12632-1-AP (LAMP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A549 using 12632-1-AP

  IP Result of anti-LAMP3 (IP:12632-1-AP, 4ug; Detection:12632-1-AP 1:500) with A549 cells lysate 2800ug.

• 

  IHC staining of human lung using 12632-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12632-1-AP (LAMP3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 12632-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12632-1-AP (LAMP3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 12632-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 12632-1-AP (LAMP3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 12632-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 12632-1-AP (LAMP3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 12632-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12632-1-AP (LAMP3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 12632-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12632-1-AP (LAMP3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A549 using 12632-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using LAMP3 antibody (12632-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, A549-Zellen, HEK-293T-Zellen, Mauslungengewebe, Maus-Thymusgewebe, Raji-Zellen, Rattenlungengewebe
• Erfolgreiche IP: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungengewebe, humanes Hodengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 4 publications below
• IF: See 9 publications below

Produktinformation

12632-1-AP bindet in WB, IP, IHC, IF, ELISA LAMP3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LAMP3 fusion protein Ag3325
• Vollständiger Name: lysosomal-associated membrane protein 3
• Berechnetes Molekulargewicht: 416 aa, 44 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC032940
• Gene symbol: LAMP3
• Gene ID (NCBI): 27074
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LAMP3 (lysosome-associated membrane protein 3), also known as DC-LAMP (DC-lysosome-associated membrane glycoprotein), TSC403 or CD208, is a highly glycosylated lysosomal membrane protein that belongs to the LAMP family. The mature glycoprotein has an apparent molecular mass of 70-90 kDa, which is considerably larger than its predicted mass of 44 kDa (PMID: 9768752). LAMP3 is expressed in lung type II pneumocytes, lymphoid organs and dendritic cells. It might change the lysosome function after the transfer of peptide-MHC class II molecules to the surface of dendritic cells. LAMP3 mRNA is up-regulated in some human cancers (PMID:9721848). LAMP3 overexpression is associated with an enhanced metastatic potential and may be a prognostic factor for cervical cancer (PMID: 16204031).

Publikationen

Species	Application	Title
human	WB	Nat Med; Haploinsufficiency leads to neurodegeneration in C9ORF72 ALS/FTD human induced motor neurons.; Authors - Yingxiao Shi
human	IF	Arthritis Rheumatol; LAMP3 induces lysosome-dependent cell death by impairing autophagic caspase-8 degradation in Sjögren's salivary glands; Authors - Hiroyuki Nakamura
monkey	WB	Nat Commun; Dstyk mutation leads to congenital scoliosis-like vertebral malformations in zebrafish via dysregulated mTORC1/TFEB pathway.; Authors - Xianding Sun
human	WB,IF	J Clin Invest; Lysosomal exocytosis of HSP70 stimulates monocytic BMP6 expression in Sjögren's syndrome.; Authors - Ying-Qian Mo
human	WB,IF	Autophagy; LAMP3 inhibits autophagy and contributes to cell death by lysosomal membrane permeabilization.; Authors - Tsutomu Tanaka
mouse	IF	Cell Prolif; Intrapulmonary distal airway stem cell transplantation repairs lung injury in chronic obstructive pulmonary disease.; Authors - Xiaofan Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ying (Verified Customer) (08-19-2019)

very good antibody

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse lung tissues

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