LATS2-Specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

LATS2-Specific Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(41)

Kat-Nr. : 20276-1-AP

Synonyme

LATS2, EC:2.7.11.1, KPM, LATS 2, Serine/threonine-protein kinase kpm

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  WB analysis using 20276-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20276-1-AP (LATS2-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20276-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20276-1-AP (LATS2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 20276-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 20276-1-AP (LATS2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 20276-1-AP

  IP result of anti-LATS2-Specific (IP:20276-1-AP, 4ug; Detection:20276-1-AP 1:300) with HEK-293 cells lysate 2320 ug.

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  IHC staining of human liver cancer using 20276-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20276-1-AP (LATS2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 20276-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20276-1-AP (LATS2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 20276-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20276-1-AP (LATS2-Specific antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon using 20276-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20276-1-AP (LATS2-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver cancer using 20276-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20276-1-AP (LATS2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 20276-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20276-1-AP (LATS2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 20276-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20276-1-AP (LATS2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 20276-1-AP

  Immunofluorescent analysis of HepG2 cells using 20276-1-AP (LATS2-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, humanes Herzgewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Kolongewebe, Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 34 publications below
• IHC: See 10 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

20276-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA LATS2-Specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: LATS, large tumor suppressor, homolog 2 (Drosophila)
• Berechnetes Molekulargewicht: 120 kDa
• Beobachtetes Molekulargewicht: 130-150 kDa
• GenBank-Zugangsnummer: NM_014572
• Gene symbol: LATS2
• Gene ID (NCBI): 26524
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LATS2, also named as KPM, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. LATS2 is a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. It negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. It is a negative regulator of the androgen receptor. LATS2 has a calculated molecular mass of 120 kDa and an apparent molecular mass of 150 kDa (PMID: 10871863). The antibody is specific to LATS2.

Publikationen

Species	Application	Title
human	WB	Mol Cancer; Co-transcriptional R-loops-mediated epigenetic regulation drives growth retardation and docetaxel chemosensitivity enhancement in advanced prostate cancer; Authors - Yufan Ying
mouse	WB	Cell Metab; Cholesterol Stabilizes TAZ in Hepatocytes to Promote Experimental Non-alcoholic Steatohepatitis.; Authors - Xiaobo Wang ; KD Validated
human	WB	Cell Death Differ; The Hippo signaling component LATS2 enhances innate immunity to inhibit HIV-1 infection through PQBP1-cGAS pathway.; Authors - Tian-Sheng He ; KO Validated
human	WB	Acta Pharmacol Sin; Gracillin suppresses cancer progression through inducing Merlin/LATS protein-protein interaction and activating Hippo signaling pathway; Authors - Jin-Xuan Su
human	WB,CoIP	Clin Transl Med; Tumor-derived exosomal miR-19b-3p facilitates M2 macrophage polarization and exosomal LINC00273 secretion to promote lung adenocarcinoma metastasis via Hippo pathway.; Authors - Jing Chen
human	WB,IHC	Oxid Med Cell Longev; miR-25 Promotes Cell Proliferation, Migration, and Invasion of Non-Small-Cell Lung Cancer by Targeting the LATS2/YAP Signaling Pathway.; Authors - Tangwei Wu